Perfringens Agar (OPSP)

Item No.  NCM0268A

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Perfringens Agar (OPSP) is used for the cultivation of anaerobic microorganisms and is not intended for use in the diagnosis of disease or other conditions in humans.

Oleandomycin, Polymixin, Sulphadiazine, Perfringens (OPSP) agar, has been used as a standard medium for Clostridium perfringens for many years. This medium was developed by Handford in 1974 to overcome some of the problems associated with enumerating Clostridium perfringens in foods. The medium is buffered and utilizes sodium metabisulphite and liver extract as sources of H2S with ferric ammonium citrate as the indicator.

The medium is made selective with the addition of X109 Sulphadiazine and X110 Oleandomycin / Polymyxin supplements. Some strains of C. perfringens may demonstrate sensitivity to the sulphadiazine antibiotic (X109) in such cases use of NCM0077, TSC Perfringens Agar Base (with X194 D-Cycloserine) should be considered.

Specifications
Analyte Clostridium perfringens
Brand NEOGEN® Culture Media
Formula
Formula Liter
Tryptone 15.0 g/L
Yeast Extract 5.0 g/L
Soy Peptone 5.0 g/L
Liver Extract 7.0 g/L
Ferric Ammonium Citrate 1.0 g/L
Sodium Metabisulphite 1.0 g/L
Tris Buffer 1.5 g/L
Agar 10.0 g/L
Grams per Litre 45.60
Litres per 500g 11.00
Mass 500 g
Package Dimensions 10.20 IN x 10.20 IN x 11.90 IN
Package Weight 15.30 LB
Plates per 500g 600.00
Preparation
  1. Suspend 45.5 grams of the medium and add to 1 liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.
  4. Allow to cool to 45-50°C before adding 2 vials each of selective supplements NCM4020-0.5* and NCM4021-0.5*, each reconstituted using 5mL of sterile deionized/RO water.

*Larger vials may be available. Please see appropriate supplement data sheet for availability and preparation instructions.

Previous Item Number Product was previously known as LabM Product: LAB109 Perfringens Agar (OPSP)

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