PALCAM Broth

SKU No.  700003093  |  Catalog No.  NCM0049A

Qty:
Minimum quantity required: 1
This item must be ordered in multiples of one case. Quantity per case: 1

PALCAM Broth is used with supplements for the selective enrichment of Listeria spp. from foods and environmental samples in a laboratory setting. PALCAM Broth is not intended for use in the diagnosis of disease or other conditions in humans.

Listeria monocytogenes, described in 1926 by Murray, Webb, and Swann, is a widespread problem in food industries. Evidence from outbreaks of listeriosis indicate the principle route of transmission is via the consumption of foodstuffs contaminated with Listeria monocytogenes. Implicated vehicles of transmission include turkey frankfurters, coleslaw, pasteurized milk, Mexican style cheese, and pate. PALCAM Broth is based on the formulation of Van Netten et al. while he was investigating the isolation of Listeria spp. from food samples. PALCAM Broth has been used for testing Listeria from raw milk.

Specifications
BrandNeogen® Culture Media
AnalyteListeria spp.
PlatformDehydrated Culture Media
Mass500 g
Formula
FormulaLiter
Peptone23.0 g/L
Yeast Extract5.0 g/L
Lithium Chloride10.0 g/L
Esculin0.8 g/L
Ferric Ammonium Citrate0.5 g/L
Mannitol5.0 g/L
Phenol Red0.08 g/L
Lecithin1.0 g/L
Polysorbate 802.0 g/L
Grams per Liter47.00
Liters per 500 g9.00
Preparation
  1. Dissolve 47 grams of the medium in one liter of purified water.
  2. Mix thoroughly.
  3. Autoclave at 121°C for 15 minutes. Cool to 45 - 50° C.
  4. Aseptically add 2 vials of NCM4041-0.5* Palcam PAC Supplement, each reconstituted using 5 mL sterile deionized/RO water.
  5. Dispense into sterile tubes.

*Larger vials may be available. Please see appropriate supplement data sheet for availability and preparation instructions.

Previous Item NumberProduct was previously known as Acumedia Product: 7370 PALCAM Broth; and LabM Product: LAB144 PALCAM Broth
Package Dimensions4.60 in x 2.90 in x 8.60 in
Package Weight1.35 lb

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