Palcam Agar Base is used with supplements as a selective and differential medium for the detection and isolation of Listeria monocytogenes from food and environmental samples. Palcam Agar Base is not intended for use in the diagnosis of disease or other conditions in humans.

Palcam Agar was developed by Van Netten et al in 1989 as an improved selective differential medium for the isolation of Listeria monocytogenes from food and environmental specimens. Improved selectivity is achieved by the combination of antibiotic supplements and microaerobic incubation. The double indicator system of esculin hydrolysis and mannitol fermentation aids differentiation of Listeria spp from enterococci and staphylococci which can be confused with Listeria spp on other types of culture media. Palcam Agar is listed within Annex E of ISO 11290-1:2017 as a suitable second selective agar.

Analyte Listeria monocytogenes
Brand NEOGEN® Culture Media
Formula Liter
Columbia Peptone Mix 23.0 g/L
Sodium Chloride 5.0 g/L
Corn Starch 1.0 g/L
Yeast Extract 3.0 g/L
Glucose 0.5 g/L
Mannitol 10.0 g/L
Esculin 0.8 g/L
Lithium Chloride 15.0 g/L
Ferric Ammonium citrate 0.5 g/L
Phenol Red 0.08 g/L
Agar 12.0 g/L
Grams per Litre 71.00
Litres per 500g 7.00
Mass 500 g
Package Dimensions 4.60 IN x 2.90 IN x 8.60 IN
Package Weight 1.35 LB
Plates per 500g 380.00
  1. Suspend 71 grams of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.
  4. Cool to 45-50°C 5. Aseptically add 2 vials of NCM4041-0.5* Palcam PAC Supplement, each reconstituted using 5 mL sterile deionized/RO water. Mix well.

*Larger vials may be available. Please see appropriate supplement data sheet for availability and preparation instructions.

Previous Item Number Product was previously known as Acumedia Product: 7669 PALCAM Agar; and LabM Product: LAB148 PALCAM Agar Base