Palcam Agar Base

SKU No.  700003280  |  Catalog No.  NCM0111A

Qty:
Minimum quantity required: 1
This item must be ordered in multiples of one case. Quantity per case: 1

Palcam Agar Base is used with supplements as a selective and differential medium for the detection and isolation of Listeria monocytogenes from food and environmental samples. Palcam Agar Base is not intended for use in the diagnosis of disease or other conditions in humans.

Palcam Agar was developed by Van Netten et al in 1989 as an improved selective differential medium for the isolation of Listeria monocytogenes from food and environmental specimens. Improved selectivity is achieved by the combination of antibiotic supplements and microaerobic incubation. The double indicator system of esculin hydrolysis and mannitol fermentation aids differentiation of Listeria spp from enterococci and staphylococci which can be confused with Listeria spp on other types of culture media. Palcam Agar is listed within Annex E of ISO 11290-1:2017 as a suitable second selective agar.

Specifications
BrandNeogen® Culture Media
AnalyteListeria monocytogenes
PlatformDehydrated Culture Media
Mass500 g
Formula
FormulaLiter
Columbia Peptone Mix23.0 g/L
Sodium Chloride5.0 g/L
Corn Starch1.0 g/L
Yeast Extract3.0 g/L
Glucose0.5 g/L
Mannitol10.0 g/L
Esculin0.8 g/L
Lithium Chloride15.0 g/L
Ferric Ammonium citrate0.5 g/L
Phenol Red0.08 g/L
Agar12.0 g/L
Grams per Liter71.00
Liters per 500 g7.00
Plates per 500 g380.00
Preparation
  1. Suspend 71 grams of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.
  4. Cool to 45-50°C 5. Aseptically add 2 vials of NCM4041-0.5* Palcam PAC Supplement, each reconstituted using 5 mL sterile deionized/RO water. Mix well.

*Larger vials may be available. Please see appropriate supplement data sheet for availability and preparation instructions.

Previous Item NumberProduct was previously known as Acumedia Product: 7669 PALCAM Agar; and LabM Product: LAB148 PALCAM Agar Base
Package Dimensions4.60 in x 2.90 in x 8.60 in
Package Weight1.35 lb

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