Nutrient Broth No. 2

Item No.  NCM0189A

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Expect availability 05/14/2021
Minimum quantity required: 1
This item must be ordered in multiples of one case. Quantity per case: 1

Nutrient Both No. 2 is used for the cultivation of fastidious bacteria, and forms the base for Preston Broth according to ISO 10272-1:2017 for the cultivation of Campylobacter spp. This media is not intended for use in the diagnosis of disease or other conditions in humans.

This broth can also be used as the suspending medium for cooked meat granules for the cultivation of anaerobic organisms.

Analyte Bacillus, Campylobacter spp., E. coli, Pseudomonas spp., Salmonella, Staphylococcus
Brand NEOGEN® Culture Media
Formula Liter
Beef Extract 10.0 g/L
Peptone 10.0 g/L
Sodium Chloride 5.0 g/L
Grams per Litre 25.00
Litres per 500g 20.00
Mass 500 g
Package Dimensions 4.60 IN x 2.90 IN x 8.60 IN
Package Weight 1.35 LB
  1. Dissolve 25 grams of the medium in one liter of purified water.
  2. Heat with frequent agitation to completely dissolve the medium if necessary.
  3. Autoclave at 121ºC for 15 minutes.
  4. When using Nutrient Broth No. 2 to prepare Preston Broth add 2 vials of X114 and 2 vials of X115. Mix well.
Previous Item Number Product was previously known as LabM Product: LAB014 Nutrient Broth No. 2



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