Muller-Kauffmann Tetrathionate-Novobiocin (MKTTn) Broth

Item No.  NCM0126A

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Muller-Kauffmann Tetrathionate-Novobiocin Broth is used for the selective and differential isolation of Salmonella spp. in a laboratory setting and is included in ISO 6579:2017. This medium is not intended for use in the diagnosis of disease or other conditions in humans. A selective enrichment medium for the detection of Salmonella spp. in food, animal feed and in environmental samples from the food production area as described in ISO 6579-1:2017. Mueller originally described a tetrathionate broth that inhibited lactose fermenting Enterobacteriaceae but not Salmonella. Kauffmann later modified the formulation to include ox bile and brilliant green to improve selectivity. Finally, Jeffries added Novobiocin to inhibit Proteus species. Meat extract and casein provide a source of nitrogen and amino acids and sodium chloride maintain the osmotic balance. Ox bile and brilliant green act as selective agents against non-target microorganisms. Tetrathionate is generated from the sodium thiosulfate. Iodine and calcium carbonate buffer the sulfuric acid generated from tetrathionate reduction. According to ISO 6579-1:2017 subculture is performed from Buffered Peptone Water (BPW) into MKTTn, followed by subculture onto Xylose-Lysine Deoxycholate (XLD) Agar ISO and second agar. This medium conforms to the performance and formulation requirements of ISO 6579-1:2017.  
Specifications
Analyte Salmonella spp.
Brand NEOGEN® Culture Media
Formula
Formula Liter
Meat Extract 4.3 g/L
Enzymatic Digest of Casein 8.6 g/L
Sodium Chloride 2.6 g/L
Calcium Carbonate 38.7 g/L
Sodium Thiosulfate (anhydrous) 30.45 g/L*
Ox Bile 4.78 g/L
Brilliant Green 0.0096 g/L

*Equivalent to 47.8g of sodium thiosulfate pentahydrate

Grams per Litre 89.40
Litres per 500g 6.00
Mass 500 g
Package Dimensions 4.60 IN x 2.90 IN x 8.60 IN
Package Weight 1.35 LB
Preparation
  1. Dissolve 89.4 grams of the medium in one liter of purified water.
  2. Mix thoroughly and bring to a boil.
  3. Cool to less than 45°C before adding supplements.
  4. Supplement NCM0126 with 20 mL of iodine-iodide solution and 4 vials of NCM4040-0.5* Novobiocin. NCM3503 is provided fully supplemented so no further additions are required.
  5. Mix well and distribute into sterile containers. Iodine-iodide solution Dissolve 25g of potassium iodide in 10 ml of water. Add 20g iodine and dilute to 100ml with sterile deionized water.

*Larger vials may be available. Please see appropriate supplement data sheet for availability and preparation instructions.

Previous Item Number Product was previously known as Acumedia Product: 9221 Muller-Kauffmann Tetrathionate-novobiocin (MKTTn) Broth; and LabM Product: LAB202 Mueller Kauffmann Tetrathionate Novobiocin Broth (MKTTn)

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