Mueller Hinton Agar II

Item No.  NCM0023A

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$119.09 each
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Mueller Hinton Agar II is used in antimicrobial susceptibility testing by the disk diffusion method. This formula conforms to Clinical and Laboratory Standard Institute (CLSI), formerly National Committee for Clinical Laboratory Standards (NCCLS).

Mueller Hinton Agar II is based on the formula recommended by Mueller and Hinton for the primary isolation of Neisseria species. Mueller and Hinton selected pea meal extract agar as a simple transparent medium containing heat stable ingredients. During their modification, starch replaced the growth-promoting properties of pea extract, acting as a “protective colloid” against toxic substances.

Bauer, Kirby, Sherris and Tuck recommended Mueller Hinton Agar for performing antibiotic susceptibility tests using a single disk of high concentration. This un-supplemented medium has been selected by the Clinical and Laboratory Standard Institute (CLSI) for several reasons. This medium is low in sulfonamide, trimethoprim and tetracycline inhibitors, and provides satisfactory growth of most non-fastidious pathogens along with demonstrating batch-to-batch reproducibility.

Mueller Hinton Agar is often abbreviated as M-H Agar and complies with requirements of the World Health Organization. Mueller Hinton Agar is specified in FDA Bacteriological Analytical Manual for food testing, and procedures commonly performed on aerobic and facultatively anaerobic bacteria. A variety of supplements can be added to Mueller Hinton Agar, including 5% defibrinated sheep or horse blood, 1% growth supplement and 2% sodium chloride.

Brand Neogen® Culture Media
Analyte Non-selective
Platform Dehydrated Culture Media
Mass 500 g
Formula Liter
Beef Extract 2.0 g/L
Acid Hydrolsate of Casein 17.5 g/L
Starch 1.5 g/L
Agar 17.0 g/L
Grams per Liter 38.00
Liters per 500 g 13.00
Plates per 500 g 720.00
  1. Suspend 38 grams of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121ºC for 15 minutes. Cool to 45-50ºC
  4. OPTIONAL: Supplement as appropriate. Pour cooled Mueller Hinton Agar II into sterile petri dishes on a level, horizontal surface to give uniform depth. Allow to cool to room temperature.
  5. Check prepared Mueller Hinton Agar to ensure the final pH is 7.3 ± 0.1 at 25ºC.
Previous Item Number Product was previously known as Acumedia Product: 7101 Mueller Hinton Agar
Package Weight 1.35 lb


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