Mueller Hinton Agar I

Item No.  NCM0036A

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Mueller Hinton Agar I is used for antimicrobial sensitivity testing by the disc diffusion method and is not intended for use in the diagnosis of disease or other conditions in humans.

A medium for antimicrobial sensitivity testing by the disc diffusion method. This medium, used in the technique of Bauer and Kirby, has been adopted by the Clinical and Laboratory Standard Institute (CLSI), formerly National Committee for Clinical Laboratory Standards (NCCLS) in the USA as the definitive method for susceptibility testing. The medium has a very low thymine and thymidine content, making it suitable for trimethoprim and sulphonamide testing, controlled to ensure correct zone sizes with aminoglycoside and tetracyline antibiotics. The medium was originally formulated as a heat labile protein free medium for the isolation of pathogenic Neisseriaceae.

Specifications
Analyte E. coli, Enterococci, Pseudomonas spp., Staphylococci
Brand NEOGEN® Culture Media
Formula
Formula Liter
Beef Extract 2.0 g/L
Acid Hydrolysed Casein 17.5 g/L
Starch 1.5 g/L
Agar 17.0 g/L
Grams per Litre 38.00
Litres per 500g 13.00
Mass 500 g
Package Dimensions 4.60 IN x 2.90 IN x 8.60 IN
Package Weight 1.35 LB
Plates per 500g 720.00
Preparation
  1. Suspend 38 grams of powder in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.
  4. Cool to 45-50°C.
Previous Item Number Product was previously known as LabM Product: LAB039 Mueller Hinton Agar

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