Modified Tryptone Soy Broth (mTSB)

Item No.  NCM0196A

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Minimum quantity required: 1
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Modified Tryptone-Soy Broth has emerged as the medium of choice for the enrichment of E. coli O157:H7 in red meats and is incorporated into ISO/TS 13136:2012. This medium is not intended for use in the diagnosis of disease or other conditions in humans.

Concern regarding E. coli O157:H7 has grown due to the severity of the disease syndromes caused, and the increase in foodborne infection, so too has the need to optimize methods for its efficient isolation. Symptoms start with severe stomach cramps and watery, bloody diarrhea, and a percentage of individuals infected will develop Haemolytic Uraemic Syndrome (HUS) leading to acute renal failure. In a comparison of four different selective broth media, MTSB was the most productive and selective for the isolation of E. coli O157:H7. mTSB is made selective for O157:H7 by including bile salts in the dehydrated medium, and the addition of novobiocin supplement (X150).

Brand NEOGEN® Culture Media
Mass 500 g
Package Dimensions 4.60 IN x 2.90 IN x 8.60 IN
Package Weight 1.35 LB
  1. Dissolve 33 grams of the powder in one liter of purified water.
  2. Heat with frequent agitation to completely dissolve the medium if necessary.
  3. Autoclave at 121°C for 15 minutes.
  4. Cool to 45-50°C and add 2 vials of NCM4040-0.5* Novobiocin Supplement, each reconstituted using 5mL of sterile deionized/RO water.

*Larger vials may be available. Please see appropriate supplement data sheet for availability and preparation instructions.

Previous Item Number Product was previously known as LabM Product: LAB165 O157 Broth (MTSB)



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