Modified Buffered Peptone Water with Pyruvate

Item No.  NCM0084A

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Modified Buffered Peptone Water with Pyruvate (mBPWp) is used for the isolation of Enterohemorrhagic E. coli (EHEC) in a laboratory setting. Modified Buffered Peptone Water with Pyruvate (mBPWp) is not intended for use in the diagnosis of disease or other conditions in humans.

Enterohemorrhagic E. coli (EHEC) are recognized as the primary cause of hemorrhagic colitis (HC) or bloody diarrhea. This infection can also lead to hemolytic uremic syndrome (HUS). Enterohemorrhagic infections are usually food or water borne and have been implicated in undercooked ground beef, raw milk, lunchmeat, water, unpasteurized apple cider, sprouts and vegetables.

Modified Buffered Peptone Water with Pyruvate is recommended in the sample preparation for the screening method following FDA/BAM Enterohemorrhagic E. coli (EHEC) Protocol. After the sample is incubated for five hours in Modified Buffered Peptone Water with Pyruvate, Cefsulodin, Acriflavin and Vancomycin are added, followed by an additional incubation period. These antimicrobic supplements effectively suppress normal flora, while allowing the growth of viable O157:H7 cells (including STEC) and is capable of detecting <1 cfu g in foods. improved recovery of pathogenic>E. coli from difficult commodities was achieved with Modified Buffered Peptone Water with Pyruvate enrichment.

Specifications
Analyte E. coli, E. coli (EHEC), Enterohemorrhagic
Brand NEOGEN® Culture Media
Formula
Formula Liter
Enzymatic Digest of Casein 10.0 g/L
Lactose 10.0 g/L
Yeast Extract 6.0 g/L
Acid Digest of Casein 5.0 g/L
Sodium Chloride 5.0 g/L
Sodium Phosphate, dibasic 3.6 g/L
Potassium Phosphate, monobasic 1.5 g/L
Sodium Pyruvate 1.0 g/L
Grams per Litre 42.10
Litres per 500g 12.00
Mass 500 g
Package Dimensions 4.60 IN x 2.90 IN x 8.60 IN
Package Weight 1.35 LB
Preparation
  1. Dissolve 42.1 grams of the medium in one liter of purified water.
  2. Mix thoroughly.
  3. Autoclave at 121ºC for 15 minutes.
Previous Item Number Product was previously known as Acumedia Product: 7736 Modified Buffered Peptone Water with Pyruvate

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