MacConkey Broth Purple

Item No.  NCM0193A

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MacConkey Broth Purple is used for the detection and enumeration of fecal coliforms (37°C) and E. coli (44°C). This medium is not intended for use in the diagnosis of disease or other conditions in humans.

The replacement of neutral red used in the original formulation by bromocresol purple makes the color change caused by acid producing organisms easier to read.

Specifications
Analyte Coliforms, E. coli, Enterobacteriaceae
Brand NEOGEN® Culture Media
Formula
Formula Liter
Peptone 20.0 g/L
Lactose 10.0 g/L
Bile Salts 5.0 g/L
Bromocresol Purple 0.01 g/L
Grams per Litre 35.00
Litres per 500g 14.00
Mass 500 g
Package Dimensions 4.60 IN x 2.90 IN x 8.60 IN
Package Weight 1.35 LB
Preparation
  1. Dissolve 35 grams of the medium in one liter of purified water.
  2. Heat with frequent agitation to completely dissolve the medium if necessary and dispense into tubes or bottles with inverted Durham tubes.
  3. Autoclave at 121°C for 15 minutes.
  4. Prepare double strength broth (70g/l) if 50ml or 10ml amounts of inoculum are to be added to equal volumes of broth. Prepare single strength broth (35g/l) if 1ml or 0.1ml amounts of inoculum are to be added to 10ml of broth.
Previous Item Number Product was previously known as LabM Product: LAB005 MacConkey Broth (Purple)

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