MacConkey Broth Purple

500 g

SKU No.  700004610  |  Catalog No.  NCM0193A

Qty:
Minimum quantity required: 1
This item must be ordered in multiples of one case. Quantity per case: 1

MacConkey Broth Purple is used for the detection and enumeration of fecal coliforms (37°C) and E. coli (44°C). This medium is not intended for use in the diagnosis of disease or other conditions in humans.

The replacement of neutral red used in the original formulation by bromocresol purple makes the color change caused by acid producing organisms easier to read.

Specifications
BrandNeogen® Culture Media
AnalyteColiforms, E. coli, Enterobacteriaceae
PlatformDehydrated Culture Media
Mass500 g
Formula
FormulaLiter
Peptone20.0 g/L
Lactose10.0 g/L
Bile Salts5.0 g/L
Bromocresol Purple0.01 g/L
Grams per Liter35.00
Liters per 500 g14.00
Preparation
  1. Dissolve 35 grams of the medium in one liter of purified water.
  2. Heat with frequent agitation to completely dissolve the medium if necessary and dispense into tubes or bottles with inverted Durham tubes.
  3. Autoclave at 121°C for 15 minutes.
  4. Prepare double strength broth (70g/l) if 50ml or 10ml amounts of inoculum are to be added to equal volumes of broth. Prepare single strength broth (35g/l) if 1ml or 0.1ml amounts of inoculum are to be added to 10ml of broth.
Previous Item NumberProduct was previously known as LabM Product: LAB005 MacConkey Broth (Purple)
Package Dimensions4.60 in x 2.90 in x 8.60 in
Package Weight1.35 lb

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