MacConkey Agar

SKU No.  700003012  |  Catalog No.  NCM0017A

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$110.00 each
$1,039.00 each
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$9,950.00 each
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Minimum quantity required: 1
This item must be ordered in multiples of one case. Quantity per case: 1
MacConkey Agar is used for the isolation and differentiation of Gram-negative enteric bacilli in a laboratory setting. Conforms to Harmonized USP/EP/JP Requirements and FDA/BAM. MacConkey Agar is not intended for use in the diagnosis of disease or other conditions in humans. A medium recommended by the Harmonized USP/EP/JP for isolation and identification of Escherichia coli from non-sterile products. Conforms to USP/EP/JP performance specification. Gelatin serves as source of carbon and nitrogen. Lactose is a fermentable carbohydrate and sodium chloride maintains the osmotic balance. Bile salts and crystal violet act as selective agents inhibiting many Gram-positive bacteria. Escherichia coli can ferment lactose to produce acid which results in a pH drop. This is indicated by neutral red resulting in pink colonies. Enough acid production will cause the precipitation of bile salts resulting in a bile precipitate or halo around lactose fermenting bacteria. Non-lactose fermenting bacteria such as Salmonella spp. grow but remain colorless with no bile precipitate. According to the Harmonized USP/EP/JP, MacConkey Broth is used as a selective enrichment broth, with subculture performed onto MacConkey Agar.
BrandNeogen® Culture Media
AnalyteE. coli
PlatformDehydrated Culture Media
Mass500 g
Enzymatic Digest of Gelatin17.0 g/L
Enzymatic Digest of Casein1.5 g/L
Enzymatic Digest of Animal Tissue1.5 g/L
Lactose10.0 g/L
Bile Salts Mixture1.5 g/L
Sodium Chloride5.0 g/L
Neutral Red0.03 g/L
Crystal Violet0.001 g/L
Agar13.5 g/L
Grams per Liter50.00
Liters per 500 g14.00
Plates per 500 g780.00
  1. Suspend 50 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121ºC for 15 minutes.
  4. Cool to 45-50°C.
Previous Item NumberProduct was previously known as Acumedia Product: 7102 MacConkey Agar; and LabM Product: HP006 MacConkey Agar (USP/EP/JP)
Package Dimensions4.60 in x 2.90 in x 8.60 in
Package Weight1.35 lb


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