m-FC Agar

Item No.  NCM0149A

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m FC Agar is used with rosolic acid for the detection and enumeration of fecal coliforms by membrane filtration in a laboratory setting. m-FC Agar is not intended for use in the diagnosis of disease or other conditions in humans.

Geldreich et al. formulated a medium to enumerate fecal coliforms (FC) using the membrane filter (m) technique without prior enrichment.

Many standard method membrane filtration procedures recommend m-FC media for testing water. The American Public Health Association (APHA) specified m-FC media and incubation at 44.5 ± 0.5°C in the fecal coliform procedure and other tests. The Association of Official Analytical Chemists (AOAC) specifies m-FC Agar for detecting total coliforms and fecal coliforms in foods. The US Environmental Protection Agency specified using m-FC media in fecal coliform methods for testing water by the direct MF method or the delayed-incubation MF methods.

Specifications
Brand Neogen® Culture Media
Platform Dehydrated Culture Media
Mass 500 g
Formula
Formula Liter
Enzymatic Digest of Casein 10.0 g/L
Enzymatic Digest of Animal Tissue 5.0 g/L
Yeast Extract 3.0 g/L
Sodium Chloride 5.0 g/L
Lactose 12.5 g/L
Bile Salts 1.5 g/L
Aniline Blue 0.1 g/L
Agar 15.0 g/L
Grams per Liter 52.00
Liters per 500 g 10.00
Preparation
  1. Suspend 52 grams of the medium in one liter of purified water containing 10 mL of 1% Rosolic Acid in 0.2 N NaOH.
  2. If necessary, adjust pH to 7.4 with 1N HCl.
  3. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  4. Cool to 45-50ºC and pour plates.
  5. DO NOT AUTOCLAVE.
Previous Item Number Product was previously known as Acumedia Product: 7397 m FC Agar
Package Dimensions 4.60 in x 2.90 in x 8.60 in
Package Weight 1.35 lb

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