Item No.  NCM0125A

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M Broth is used for the cultivation of Salmonella spp in a laboratory setting. M-Broth is not intended for use in the diagnosis of disease or other conditions in humans.

M-Broth is prepared according to the formula of Sperber and Diebel, and contains the nutrients necessary for good growth and flagella development of Salmonella. This medium is used for cultivating Salmonella in foods and feeds by the accelerated enrichment serology (ES) procedure. M-Broth conforms to standard procedures recommended in food testing and monoclonal and polyclonal enzyme immunoassays (EIA).

Brand NEOGEN® Culture Media
Formula Liter
Enzymatic Digest of Casein 12.5 g/L
Yeast Extract 5.0 g/L
D-Mannose 2.0 g/L
Sodium Citrate 5.0 g/L
Sodium Chloride 5.0 g/L
Potassium Phosphate 5.0 g/L
Manganese Chloride 0.14 g/L
Magnesium Sulfate 0.8 g/L
Ferrous Sulfate 0.04 g/L
Polysorbate 80 0.75 g/L
Mass 500 g
Package Dimensions 4.60 IN x 2.90 IN x 8.60 IN
Package Weight 1.35 LB
  1. Dissolve 36.2 g of the medium in one liter of purified water.
  2. Mix thoroughly.
  3. Autoclave at 121ºC for 15 minutes.
Previous Item Number Product was previously known as Acumedia Product: 7296 M-Broth