Lysine Iron Agar

Item No.  NCM0140A

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Minimum quantity required: 1
This item must be ordered in multiples of one case. Quantity per case: 1
Lysine Iron Agar is used for the differentiation of microorganisms on the basis of lysine decarboxylase and hydrogen sulfide production. Lysine Iron Agar is not intended for use in the diagnosis of disease or other conditions in humans. Lysine Iron Agar is prepared according to the formulation of Edwards and Fife, who developed the medium to detect Salmonella arizonae. S. arizonae ferments lactose rapidly, and the authors found expected H2S production on Triple Sugar Iron Agar was suppressed. Detection of S. arizonae is important because it has been implicated in food borne infections. By eliminating lactose and incorporating lysine, Edwards and Fife devised a medium differentiating enteric bacilli based on their ability to decarboxylate or deaminate lysine and produce abundant hydrogen sulfide. This medium is recommended for detecting rapid lactose-fermenting S. arizonae.  
Analyte Enteric Pathogens
Brand NEOGEN® Culture Media
Formula Liter
Enzymatic Digest of Gelatin 5.0 g/L
Yeast Extract 3.0 g/L
Dextrose (Glucose) 1.0 g/L
L-Lysine 10.0 g/L
Ferric Ammonium Citrate 0.5 g/L
Sodium Thiosulfate 0.04 g/L
Bromocresol Purple 0.02 g/L
Agar 13.5 g/L*

*10 -15 g according to gel strength

Grams per Litre 32.00
Litres per 500g 16.00
Mass 500 g
Package Dimensions 4.60 IN x 2.90 IN x 8.60 IN
Package Weight 1.35 LB
Plates per 500g 870.00
  1. Suspend 33 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Dispense into test tubes and autoclave at 121°C for 15 minutes.
  4. After autoclaving, allow medium to solidify in a slanted position.
Previous Item Number Product was previously known as Acumedia Product: 7211 Lysine Iron Agar; and LabM Product: LAB054 Lysine Iron Agar



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