Listeria Enrichment Broth

Item No.  NCM0055A

This item has shipping restrictions.
$87.00 each
$795.00 each
$1,616.00 each
$7,850.00 each
In stock
Out of stock
Expect availability in 2-4 weeks
Qty:
Minimum quantity required: 1
This item must be ordered in multiples of one case. Quantity per case: 1

Listeria Enrichment Broth is used for selective enrichment of Listeria spp and is not intended for use in the diagnosis of disease or other conditions in humans.

Listeria monocytogenes, first described in 1926 by Murray, Webb, and Swann, is an extensive problem in public health and food industries. This organism has the ability to cause human illness and death, particularly in immunocompromised individuals and pregnant women. Epidemiological evidence from outbreaks of listeriosis has indicated that the principle route of transmission is via consumption of foodstuffs contaminated with Listeria monocytogenes. Implicated vehicles of transmission include turkey frankfurters, coleslaw, pasteurized milk, Mexican style cheese, and pate. Listeria spp. are ubiquitous in nature, being present in a wide range of unprocessed foods as well as in soil, sewage, and river water.

Listeria Enrichment Broth is based on the formula developed by Lovett et al. in which Tryptic Soy Broth is supplemented with Yeast Extract for optimum growth. Listeria spp. grow over a pH range of 5.0 - 9.6, and survive in food products with pH levels outside these parameters. Listeria spp. are microaerophilic, Gram-positive, asporogenous, non-encapsulated, non-branching, short, motile rods. Motility is pronounced at 20°C. Identification of Listeria is based on successful isolation of the organism, biochemical characterization, and serological confirmation.

Specifications
Brand NEOGEN® Culture Media
Analyte Listeria spp.
Platform Dehydrated Culture Media
Mass 500 g
Formula
Formula Liter
Enzymatic Digest of Casein 17.0 g/L
Peptone Mix 3.0 g/L
Yeast Extract 6.0 g/L
Dextrose 2.5 g/L
Sodium Chloride 5.0 g/L
Dipotassium Phosphate 2.5 g/L
Cycloheximide 0.05 g/L
Acriflavin 0.015 g/L
Nalidixic Acid 0.04 g/L
Grams per Liter 36.10
Liters per 500 g 14.00
Preparation
  1. Dissolve 36.1 g of the medium in one liter of purified water.
  2. Mix thoroughly.
  3. Autoclave at 121°C for 15 minutes.
Previous Item Number Product was previously known as Acumedia Product: 7398 Listeria Enrichment Broth
Package Dimensions 4.60 in x 2.90 in x 8.60 in
Package Weight 1.35 lb

Support

Validation

Our experts are here to help. If you need information beyond what is provided in Validation Reports under the Documents tab, please contact us and we will be happy to assist you. Learn more