Letheen Broth Base Modified without Tween

Item No.  NCM0129A

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Letheen Broth Base Modified without Tween is used with Polysorbate 80 for the recovery of microorganisms from cosmetics in a laboratory setting. Letheen Broth Base Modified without Tween is not intended for use in the diagnosis of disease or other conditions in humans.

In 1948, Weber and Black described the value of a highly nutritional solid medium containing neutralizing agents for quaternary ammonium compounds in sanitizers. The addition of Lecithin and Polysorbate 80 to Tryptone Glucose Extract (TGE) Agar resulted in a medium that effectively neutralizes quaternary ammonium compounds in testing of germicidal activity. Total neutralization of disinfectants is critical. Disinfectant residues can result in a false negative (no-growth) test.

Letheen Broth Base Modified without Tween is based on the formula described in FDA Bacteriological Analytical Manual, and a modification of Letheen Broth Base. Letheen Broth Base Modified is recommended by the FDA for use in the microbiological testing of cosmetics.

Analyte Non-selective
Brand NEOGEN® Culture Media
Formula Liter
Letheen Broth Base 20.7 g/L
Enzymatic Digest of Casein 5.0 g/L
Enzymatic Digest of Animal Tissue 10.0 g/L
Yeast Extract 2.0 g/L 2.0 g/L
Sodium Bisulfite 0.1 g/L
Grams per Litre 37.80
Litres per 500g 13.00
Mass 500 g
Package Dimensions 4.60 IN x 2.90 IN x 8.60 IN
Package Weight 1.35 LB
  1. Dissolve 37.8 g of the medium and 5 g of Tween 80 (7992) in one liter of purified water.
  2. Heat with frequent agitation to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.
Previous Item Number Product was previously known as Acumedia Product: 7496 Letheen Broth Base, Modified



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