Letheen Agar Base Modified without Tween

Item No.  NCM0130A

This item has shipping restrictions.
In stock
Out of stock
Expect availability in 2-4 weeks
Minimum quantity required: 1
This item must be ordered in multiples of one case. Quantity per case: 1

Letheen Agar Base Modified (without Tween) is used with Tween 80 (Polysorbate 80) for the isolation of microorganisms from cosmetics in a laboratory setting. Letheen Agar Base Modified is not intended for use in the diagnosis of disease or other conditions in humans.

In 1948, Weber and Black described the value of a highly nutritional solid medium containing neutralizing agents for quaternary ammonium compounds in sanitizers. The addition of Lecithin and Tween 80 (Polysorbate 80) to Tryptone Glucose Extract (TGE) Agar resulted in a medium that effectively neutralizes quaternary ammonium compounds in testing of germicidal activity. Total neutralization of disinfectants is critical. Disinfectant residues can result in a false negative (no-growth) test.

Letheen Agar Base Modified is based on the formula described in FDA Bacteriological Analytical Manual, and a modification of Letheen Agar Base. Letheen Agar Base Modified is recommended by the FDA for use in the microbiological testing of cosmetics.

Analyte Non-selective
Brand NEOGEN® Culture Media
Formula Liter
Letheen Agar Base 25.0 g/L
Enzymatic Digest of Casein 10.0 g/L
Enzymatic Digest of Animal Tissue 10.0 g/L
Yeast Extract 2.0 g/L
Sodium Chloride 5.0 g/L
Sodium Bisulfite 0.1 g/L
Grams per Litre 52.10
Litres per 500g 10.00
Mass 500 g
Package Dimensions 4.60 IN x 2.90 IN x 8.60 IN
Package Weight 1.35 LB
Plates per 500g 520.00
  1. Suspend 52.1 g of the medium and 7 mL of Tween 80 (Polysorbate 80) in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.
  4. Cool to 45-50°C.
Previous Item Number Product was previously known as Acumedia Product: 7496 Letheen Broth Base, Modified



Our experts are here to help. If you need information beyond what is provided in Validation Reports under the Documents tab, please contact us and we will be happy to assist you. Learn more