LB Agar, Miller

Item No.  NCM0142A

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LB Agar (Miller), a nutritionally rich medium designed for growth of pure cultures of recombinant strains, is based on the formulation described by Miller. E. coli is grown to late log phase in LB Medium. Some plasmid vectors replicate to a high copy number and do not require selective amplification. Some vectors do not replicate so freely and need to be selectively amplified. Chloramphenicol may be added to inhibit host synthesis and prevent replication of the bacterial chromosome.

LB Agar, Miller contains 10 g/L of sodium chloride, different from the levels in Lennox formulations. This allows the researcher to select the optimal salt concentration for a specific strain. The medium may be aseptically supplemented with glucose or antibiotics.

Analyte Non-selective
Brand NEOGEN® Culture Media
Formula Liter
Tryptone 10.0 g/L
Yeast Extract 5.0 g/L
Sodium Chloride 10.0 g/L
Agar 15.0 g/L
Grams per Litre 40.00
Litres per 500g 13.00
Mass 500 g
Package Dimensions 4.60 IN x 2.90 IN x 8.60 IN
Package Weight 1.35 LB
Plates per 500g 680.00
  1. Suspend 40 grams of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.
  4. Cool to 45-50°C and aseptically add filter sterilized antibiotic if required.
Previous Item Number Product was previously known as Acumedia Product: 7213 LB Agar (Miller); and LabM Product: LAB168 LB Agar (Miller)