Lactobacillus Selective Agar Base

Item No.  NCM0275A

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Minimum quantity required: 1
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Lactobacillus Selective Agar Base is used for the isolation and enumeration of lactobacilli in a laboratory setting. Lactobacillus Selective Agar Base is not intended for use in the diagnosis of disease or other conditions in humans.

Lactobacillus Selective Agar Base was developed by Rogosa, Mitchell, and Wiseman. This medium is used for isolation, enumeration, and identification of lactobacilli in foods. The low pH and high acetate concentrations effectively suppress other bacterial flora allowing lactobacilli to flourish.

Analyte Lactobacillus spp.
Brand NEOGEN® Culture Media
Formula Liter
Enzymatic Digest of Casein 10.0 g/L
Yeast Extract 5.0 g/L
Monopotassium Phosphate 6.0 g/L
Ammonium Citrate 2.0 g/L
Dextrose 20.0 g/L
Sodium Acetate 25.0 g/L
Magnesium Sulfate 0.575 g/L
Manganese Sulfate 0.12 g/L
Ferrous Sulfate 0.034 g/L
Polysorbate 80 1.0 g/L
Agar 15.0 g/L
Grams per Litre 84.00
Litres per 500g 6.00
Mass 500 g
Package Dimensions 10.20 IN x 10.20 IN x 11.90 IN
Package Weight 15.30 LB
Plates per 500g 320.00
  1. Suspend 84 g of the medium in one liter of purified water. Mix thoroughly.
  2. Add 1.32 mL of glacial acetic acid.
  3. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  4. Avoid overheating. DO NOT AUTOCLAVE.
Previous Item Number Product was previously known as Acumedia Product: 7234 Lactobacillus Selective Agar Base

Additional Information

Supplement: Glacial Acetic Acid, 1.32 mL



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