Lactobacilli MRS Agar

Item No.  NCM0035A

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Lactobacilli MRS Agar is used for the cultivation of lactobacilli and is not intended for use in the diagnosis of disease or other conditions in humans. MRS Agar is a medium for the cultivation and enumeration of Lactobacillus spp. Originally developed in 1960 by de Man, Rogosa & Sharpe, the medium is suitable for most lactic acid bacteria and is intended as a substitute for Tomato Juice Agar. When acidified to pH 5.4 MRS Agar can be used to enumerate Lactobacillus bulgaricus in yogurts. Nutrition is provided by a mixture of carefully selected peptones, glucose, beef & yeast extracts while Tween® 80, magnesium and manganese sulfates act as growth stimulants. Selectivity against streptococci & molds is provided by ammonium citrate and sodium acetate. Used at low pH, ammonium citrate allows growth of lactobacilli while inhibiting a number of other microorganism groups. Occasionally, sterilization of this medium at 121°C for 15 minutes, in some autoclaves, may cause the pH to fall outside of the specified pH limits 6.4 +/- 0.2. In these rare cases, adjustment of the medium using acetic acid or sodium hydroxide is recommended.
Specifications
Brand Neogen® Culture Media
Analyte Lactobacillus spp.
Platform Dehydrated Culture Media
Mass 500 g
Formula
Formula Liter
Peptone 10.0 g/L
Yeast Extract 5.0 g/L
Beef Extract 10.0 g/L
Glucose 20.0 g/L
Dipotassium Phosphate 2.0 g/L
Sodium Acetate 5.0 g/L
Triammonium Citrate 2.0 g/L
Magnesium Sulfate 0.2 g/L
Manganese Sulfate 0.05 g/L
Tween 80 1.08 g/L
Agar 15.0 g/L
Grams per Liter 70.00
Liters per 500 g 7.00
Plates per 500 g 390.00
Preparation
  1. Suspend 70 grams of the medium in one liter of purified water.
  2. Heat with frequent agitation to completely dissolve the medium, if necessary.
  3. Autoclave at 121°C for 15 minutes.
  4. Cool to 45-50°C.
  5. If acidified medium is required, adjust pH aseptically prior to pouring.
Previous Item Number Product was previously known as Acumedia Product: 7543 Lactobacilli MRS Agar; and LabM Product: LAB093 M.R.S Agar - de Man, Rogosa and Sharpe Agar
Package Weight 1.35 lb

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