Lactobacilli MRS Agar

SKU No.  700003065  |  Catalog No.  NCM0035A

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Lactobacilli MRS Agar is used for the cultivation of lactobacilli and is not intended for use in the diagnosis of disease or other conditions in humans. MRS Agar is a medium for the cultivation and enumeration of Lactobacillus spp. Originally developed in 1960 by de Man, Rogosa & Sharpe, the medium is suitable for most lactic acid bacteria and is intended as a substitute for Tomato Juice Agar. When acidified to pH 5.4 MRS Agar can be used to enumerate Lactobacillus bulgaricus in yogurts. Nutrition is provided by a mixture of carefully selected peptones, glucose, beef & yeast extracts while Tween® 80, magnesium and manganese sulfates act as growth stimulants. Selectivity against streptococci & molds is provided by ammonium citrate and sodium acetate. Used at low pH, ammonium citrate allows growth of lactobacilli while inhibiting a number of other microorganism groups. Occasionally, sterilization of this medium at 121°C for 15 minutes, in some autoclaves, may cause the pH to fall outside of the specified pH limits 6.4 +/- 0.2. In these rare cases, adjustment of the medium using acetic acid or sodium hydroxide is recommended.
Specifications
BrandNeogen® Culture Media
AnalyteLactobacillus spp.
PlatformDehydrated Culture Media
Mass500 g
Formula
FormulaLiter
Peptone10.0 g/L
Yeast Extract5.0 g/L
Beef Extract10.0 g/L
Glucose20.0 g/L
Dipotassium Phosphate2.0 g/L
Sodium Acetate5.0 g/L
Triammonium Citrate2.0 g/L
Magnesium Sulfate0.2 g/L
Manganese Sulfate0.05 g/L
Tween 801.08 g/L
Agar15.0 g/L
Grams per Liter70.00
Liters per 500 g7.00
Plates per 500 g390.00
Preparation
  1. Suspend 70 grams of the medium in one liter of purified water.
  2. Heat with frequent agitation to completely dissolve the medium, if necessary.
  3. Autoclave at 121°C for 15 minutes.
  4. Cool to 45-50°C.
  5. If acidified medium is required, adjust pH aseptically prior to pouring.
Previous Item NumberProduct was previously known as Acumedia Product: 7543 Lactobacilli MRS Agar; and LabM Product: LAB093 M.R.S Agar - de Man, Rogosa and Sharpe Agar
Package Dimensions4.60 in x 2.90 in x 8.60 in
Package Weight1.35 lb

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