NEO-GRID (Sterile) 36-Grid

Item No.  6866

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$212.10 each
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NEO-GRID® disposable units and ISO-GRID® membranes are filtration systems used on food, beverage and water samples for the detection, confirmation and enumeration of microorganisms including yeast and mold, total plate counts, coliforms, generic E. coli, E. coli O157:H7, Salmonella, Listeria, Listeria monocytogenes, Staphylococcus and more. ISO-GRID/NEO-GRID tests are based on the concept of hydrophobic grid membrane filtration. The filter membrane is embossed with hydrophobic ink to produce a grid containing 1600 individual squares. The ink’s hydrophobic nature contains the growth of an organism isolated during the filtration process within the square of capture.
Analyte E. coli O157, Coliforms, E. coli, Listeria, Listeria monocytogenes, Salmonella, Total Plate Count, Yeast & Mold

    ISO-GRID/NEO-GRID Method Approvals

    Yeast and Mold Count AOAC No. 995.21
    Total Bacterial Count AOAC No. 986.32
    Total Coliform/E. coli Count AOAC No. 990.11
    Salmonella Detection AOAC No. 991.12
    E. coli O157:H7 Count AOAC No. 997.11

    Canadian Compendium of Analytical Methods-Health Canada

    Enumeration of coliforms in foods by the hydrophobic grid membrane filter method. MFHPB-17
    Enumeration of E. coli in foods by the hydrophobic grid membrane filter method. MFHPB-26
    Enumeration of fecal coliforms in foods by the hydrophobic grid membrane filter method. MFLP-55
    Determination of aerobic colony count in foods by the hydrophobic grid membrane filter method. MFLP-56
    Screening bottled water for the presence of indicator and pathogenic bacteria. MFLP-60

    Canadian Standard Methods for the Examination of Water and Wastewater

    Standard total coliform membrane filter procedure 9222B
    Fecal coliform membrane filter procedure 9222D

Package Weight 7.40 lb

Additional Information

Culture Media

Product # Product Description
6903A BMA Agar – For the confirmation of E. coli.
6901A EF-18 Agar – For detecting and confirming Salmonella.
6913A LM-137 Agar – For the presumptive enumeration of Listeria and Listeria monocytogenes.
6902A LMG Agar – For the detection of coliforms.
6910A SCCRAM Broth – For the enrichment of Salmonella.
6907A SD-39 Agar – For the enumeration of E. coli and E. coli O157:H7.
6905A TSAF Agar – Used to determine total bacterial counts.
6904A YM-11 Agar – For the enumeration of yeasts and molds in only 48 hours.


Enzymes are used to digest the food commodities so that they can pass through the 0.45 µm membrane. The following enzymes are available:

Product # Product Description
6852E AMG – For the digestion of starchy foods.
6851E APUG – For the digestion of proteinaceous foods.
6853E Cellulase – For the digestion of gums.
6854E Hemicellulase – For the digestion of gums.
6850E Papain – For the digestion of proteinaceous foods.


Product # Product Description
6810 1-Place Filtration Manifold
6800 3-Place Filtration Manifold
6801 6-Place Filtration Manifold
6821 Rubber Tubing (10 feet)
6822 Side-Arm Flask Assembly (1 L)
6824 Vacuum Pump - ¼ hp (Gast)
6818 Vacuum Pump Oil (quart)
6828 Forceps
6812 Vacuum Tubing Assembly
6823 Tubing Clamp


Samples are first diluted in a sterile buffer. The ISO-GRID /NEO-GRID filtration unit then is placed onto a manifold connected to a vacuum source, and a sterile filter membrane is placed in the ISO-GRID filtration unit (NEO-GRID already contains a sterile filter membrane). Using the vacuum source, a portion of the diluted sample is filtered through the filter membrane.

The membrane is transferred to an agar plate prepared with a medium formulated to promote/differentiate the growth of the target organism. Following incubation, the plate’s squares containing presumptively positive colonies are counted to yield a most probable number (MPN) result.


Our customers’ success is our shared success. Our experts are ready to train you and your team on our solutions, so you can rest easy knowing procedures are performed properly and yield accurate results. In addition, we provide certificates upon completion of training to provide you with the documentation needed for audit traceability.



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