Harlequin® mLGA

Item No.  NCM1009S

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Membrane Lactose Glucuronide Agar (mLGA) is a medium for the detection and identification of E. coli and coliforms from water samples and is not intended for use in the diagnosis of disease or other conditions in humans. Traditionally, membrane Lauryl Sulphate Broth (mLSB) has been used as the standard media for isolating coliforms (including E. coli) from water potentially contaminated with sewage. Harlequin membrane Lactose Glucuronide Agar (mLGA) is a modification of mLSB aimed at reducing costs by reducing the number of filters used per test sample and aiding in the recovery and identification of coliforms and E. coli. The medium has been modified from the mLSB formulation by the incorporation of X-glucuronide (BCIG), sodium pyruvate and agar. X-glucuronide is a chromogenic substrate which detects the β-glucuronidase enzyme - highly specific for E. coli* - and allows for the presumptive isolation of E. coli. Sodium pyruvate aids the recovery of chlorine stressed organisms and agar is incorporated to remove the need for absorbent pads. This medium is recommended for the enumeration of coliform bacteria and E. coli by a single membrane filtration technique in The Microbiology of Drinking Water 2009 (previously Report 71). *96-97% of E. coli strains positive. A notable exception is E. coli O157:H7.
Analyte Coliforms, E. coli
Brand NEOGEN® Culture Media
Formula Liter
Peptone 40.0 g/L
Yeast Extract 6.0 g/L
Lactose 30.0 g/L
Phenol Red 0.2 g/L
Sodium Lauryl Sulphate 1.0 g/L
Sodium Pyruvate 0.5 g/L
X-Glucuronide (BCIG) 0.2 g/L
Agar 10.0 g/L
Grams per Litre 88.00
Litres per 500g 6.00
Mass 88 g
Package Dimensions 2.76 IN x 2.76 IN x 3.74 IN
Package Weight 0.31 LB
Plates per 500g 310.00
  1. Suspend 88 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 115ºC for 10 minutes.
  4. Cool to 45-50°C.
Previous Item Number Product was previously known as LabM Product: HAL009 Harlequin mLGA



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