Harlequin® Listeria Chromogenic Agar (Ottaviani & Agosti) (LCA)

Item No.  700004794  |  Catalog No.  NCM1004S

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Harlequin® Listeria Chromogenic Agar (according to the formulation of Ottaviani and Agosti) is a selective medium for the isolation and presumptive identification of Listeria monocytogenes from foodstuffs and related materials as described in ISO 11290-1:2017. This medium is not intended to diagnose diseases or other conditions in humans.

Lithium chloride in the base medium and supplementary antimicrobial compounds ceftazidime, polymyxin, nalidixic acid, and cycloheximide provide the medium's selectivity. Chromogenic activity is a result of a chromogenic substrate for the detection of the β-glucosidase enzyme, common to all Listeria spp. and a few strains of Enterococci and Bacilli.

The specific differential activity of this agar is obtained with a proprietary lecithin substrate for the detection of the phospholipase enzyme present in the L. monocytogenes colonies growing on this media. This enzyme activity will result in a halo of precipitation surrounding the target colonies.

Combining both the chromogenic and phospholipase enzyme reactions makes it possible to differentiate Listeria monocytogenes (blue colonies surrounded by an opaque halo) from other Listeria spp. (blue colonies without an opaque halo).

LCA Agar forms part of Neogen's innovative One Broth One Plate Listeria (OBOP-L) workflow that has been validated according to ISO 16140-2 and certified by AFNOR.

Specifications
Brand Neogen® Culture Media
Analyte Listeria spp.
Platform Dehydrated Culture Media, One Broth One Plate
Result Type Presence/Absence
Approvals This product forms part of Neogen's innovative One Broth One Plate Listeria (OBOP-L) workflow that has been validated according to ISO 16140-2 and certified by AFNOR.
Formula
Formula Liter
Meat Peptone 18.0 g/L
Tryptone 6.0 g/L
Yeast Extract 10.0 g/L
Lithium Chloride 10.0 g/L
Sodium Chloride 5.0 g/L
Disodium Hydrogen Orthophosphate Anhydrous 2.5 g/L
Sodium Pyruvate 2.0 g/L
Glucose 2.0 g/L
Glycerophosphate 1.0 g/L
Magnesium Sulphate 0.5 g/L
5-Bromo-4-Chloro-3Indolyl-β-d-Glucopyranoside 0.05 g/L
Agar 13.5 g/L
Grams per Liter 70.50
Liters per 500 g 7.00
Plates per 500 g 390.00
Preparation
  1. Suspend 70.5 g of the medium in 950 mL of purified water.
  2. Heat with frequent agitation and boil for 1 minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.
  4. Cool to 45–50°C and add 2 vials of reconstituted NCM4002-0.5* supplement, each reconstituted using 5 mL of sterile deionized/RO water. Swirl to mix.
  5. Add 2 vials of NCM4001-0.5* supplement (preheated to 48–50°C). Mix well with gentle end-overend mixing.

*Larger vials may be available. Please see the appropriate supplement data sheet for availability and preparation instructions.

Previous Item Number Product was previously known as LabM Product: HAL010 Harlequin® Listeria Chromogenic Agar
Storage Conditions 2-8 °C away from direct sunlight
Package Dimensions 2.76 in x 2.76 in x 3.74 in
Package Weight 0.31 lb

Documents

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Harlequin® Listeria Chromogenic Agar according to Ottaviani & Agosti - English - US Harlequin® Listeria Chromogenic Agar (Ottaviani & Agosti) (LCA) - Inglês - UE/Reino Unido Harlequin® Listeria Chromogenic Agar according to Ottaviani & Agosti - Chinese Simplified - China Harlequin® Listeria Chromogenic Agar according to Ottaviani & Agosti - Chinese Traditional - Taiwan Harlequin® Listeria Chromogenic Agar according to Ottaviani & Agosti - Danish - Denmark Harlequin® Listeria Chromogenic Agar according to Ottaviani & Agosti - Dutch - Netherlands Harlequin® Listeria Chromogenic Agar according to Ottaviani & Agosti - English - Australia Harlequin® Listeria Chromogenic Agar according to Ottaviani & Agosti - English - Canada Harlequin® Listeria Chromogenic Agar according to Ottaviani & Agosti - English - Great Britain Harlequin® Listeria Chromogenic Agar according to Ottaviani & Agosti - English - New Zealand Harlequin® Listeria Chromogenic Agar according to Ottaviani & Agosti - English - Philippines Harlequin® Listeria Chromogenic Agar according to Ottaviani & Agosti - Finnish - Finland Harlequin® Listeria Chromogenic Agar according to Ottaviani & Agosti - French Harlequin® Listeria Chromogenic Agar according to Ottaviani & Agosti - French - Canada Harlequin® Listeria Chromogenic Agar according to Ottaviani & Agosti - German Harlequin® Listeria Chromogenic Agar according to Ottaviani & Agosti - Hungarian - Hungary Harlequin® Listeria Chromogenic Agar according to Ottaviani & Agosti - Indonesian - Indonesia Harlequin® Listeria Chromogenic Agar according to Ottaviani & Agosti - Italian - Italy Harlequin® Listeria Chromogenic Agar according to Ottaviani & Agosti - Japanese - Japan Harlequin® Listeria Chromogenic Agar according to Ottaviani & Agosti - Korean - Korea Harlequin® Listeria Chromogenic Agar according to Ottaviani & Agosti - Malay - Malaysia Harlequin® Listeria Chromogenic Agar according to Ottaviani & Agosti - Norwegian - Norway Harlequin® Listeria Chromogenic Agar according to Ottaviani & Agosti - Polish - Poland Harlequin® Listeria Chromogenic Agar according to Ottaviani & Agosti - Portuguese - Brazil Harlequin® Listeria Chromogenic Agar according to Ottaviani & Agosti - Spanish - Mexico/Central America Harlequin® Listeria Chromogenic Agar according to Ottaviani & Agosti - Spanish - South America Harlequin® Listeria Chromogenic Agar according to Ottaviani & Agosti - Spanish - US Harlequin® Listeria Chromogenic Agar according to Ottaviani & Agosti - Swedish - Sweden Harlequin® Listeria Chromogenic Agar according to Ottaviani & Agosti - Thai - Thailand Harlequin® Listeria Chromogenic Agar according to Ottaviani & Agosti - Vietnamese - Vietnam

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