Harlequin® Chromogenic Agar for Salmonella Esterase (CASE)

Item No.  NCM1006S

A selective culture media designed for the isolation of Salmonella spp..

This product forms part of NEOGEN’s innovative One Broth One Plate for Salmonella (OBOP-S) workflow that has been validated according to ISO 16140-2 and certified by both MicroVal and AOAC.

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CASE is a selective chromogenic agar for the detection of Salmonella that can be used as part of Neogens's Salmonella solution and ISO 6579-1:2017 workflow. It is not intended for use in the diagnosis of disease or other conditions in humans. CASE utilizes a dual chromogenic system to differentiate between Salmonella and non-target organisms that grow on the agar. The first chromogen is a target for esterase activity present in all Salmonella species, and results in blue/green colonies. The second chromogen is a target for ß-glucosidase activity, present in non-target organisms that are able to grow on the agar and also possess esterase activity. Utilization of the second chromogen masks the presentation of the first, resulting in black colonies in non-Salmonella organisms. All other organisms are either inhibited or grow colorless on the agar. The medium contains an opacity agent that provides a white background, facilitating easy reading and identification. The media is able to detect non-motile Salmonella (S. Pullorum and S. Gallinarum) as well as monophasic variants (1,4,[5],12:i:-). The media is also able to detect serovars that traditionally present weak esterase activity (S. Dublin), and lactose positive strains (S. Arizonae). According to ISO 6579-1:2017, subculture is performed separately from both Rappaport-Vassiliadis medium with Soya (RVS broth) and Muller-Kauffmann tetrathionate-novobiocin (MKTTn) broth.
Specifications
Analyte Salmonella spp.
Approvals

This product forms part of NEOGEN’s innovative One Broth One Plate for Salmonella (OBOP-S) workflow that has been validated according to ISO 16140-2 and certified by both MicroVal and AOAC.

Brand NEOGEN® Culture Media
Formula
Formula Liter
Growth Mix 10.5 g/L
Selective Mix 11.0 g/L
Buffer 7.0 g/L
Opacity Agents 7.5 g/L
Chromogenic Mix 1.4 g/L
Agar 12.5 g/L
Grams per Litre 49.90
Litres per 500g 10.00
Mass 49.9 g
Package Dimensions 2.76 IN x 2.76 IN x 3.74 IN
Package Weight 0.31 LB
Plates per 500g 540.00
Platform Dehydrated Culture Media, One Broth, One Plate
Preparation
  1. Suspend 49.9 grams of medium in one liter of purified water.
  2. Bring rapidly to the boil with frequent agitation and temper in a water bath to 45-50°C.
Previous Item Number Product was previously known as LabM Product: HAL015 Harlequin® Chromogenic Agar for Salmonella Esterase (CASE)
Result Type Presence/Absence
Storage Conditions 2-8 °C away from direct sunlight

Additional Information

CASE Agar forms part of NEOGEN's innovative One Broth One Plate for Salmonella (OBOP-S) workflow. The OBOP-S workflow has the following external validations and certifications: AOAC RI Validation PTM 102002 and MicroVal Validation Certification 2019LR88

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Validation

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