Fraser Broth
SKU No.  700003097  | Catalog No.  NCM0050A
Top

Fraser Broth

SKU No.  700003097  |  Catalog No.  NCM0050A

Learn more
Qty:
Minimum quantity required: 1
This item must be ordered in multiples of one case. Quantity per case: 1
To order or for more information contact us
To order or for more information contact us

Fraser Broth is a selective medium used for the secondary enrichment of Listeria monocytogenes and Listeria spp., as described in ISO 11290-1:2017. Fraser Broth is not intended for use in the diagnosis of disease or other conditions in humans.

Fraser Broth was developed as a modification of UVM medium (NCM0012) and is based on the formula as described by Fraser and Sperber. Listeria spp. hydrolyze the esculin to esculetin which reacts with the ferric ammonium citrate (X211 or 7984) resulting in a black precipitate and a visible positive reaction.

Specifications
BrandNeogen® Culture Media
AnalyteListeria spp.
PlatformDehydrated Culture Media
Mass500 g
Formula
FormulaLiter
Enzymatic Digest of Animal Tissues5.0 g/L
Enzymatic Digest of Casein5.0 g/L
Meat Extract5.0 g/L
Yeast Extract5.0 g/L
Esculin1.0 g/L
Disodium Hydrogen Phosphate9.6 g/L*
Potassium Dihydrogen Phosphate1.35 g/L
Sodium Chloride20.0 g/L
Lithium Chloride3.0 g/L
Acriflavine0.025 g/L
Nalidixic Acide0.02 g/L
*Equivalent to Disodium Hydrogen Phosphate Dihydrate 12.0 g/L
Grams per Liter55.00
Liters per 500 g9.00
Preparation
  1. Dissolve 55 grams of the medium in 1 liter of purified water.
  2. Mix thoroughly.
  3. Autoclave at 121°C for 15 minutes.
  4. Cool to 45-50°C.
  5. Aseptically add 2 vials of NCM4009-0.5* Ferric Ammonium Citrate Supplement and mix thoroughly before dispensing.

*Larger vials may be available. Please see appropriate supplement data sheet for availability and preparation instructions.

Previous Item NumberProduct was previously known as Acumedia Product: 7502 Fraser Broth Base; and LabM Product: LAB212 Fraser Broth PLUS
Package Dimensions4.60 in x 2.90 in x 8.60 in
Package Weight1.35 lb

Additional Information

Ferric Ammonium Citrate Supplement (X211) or Fraser broth base supplement (7984) may be used

Documents

To access certificates, please use our technical document search page.

Fraser Broth

Fraser Broth - English - US Fraser Broth - Bulgarian - Bulgaria Fraser Broth - Chinese Simplified - China Fraser Broth - Chinese Traditional - Taiwan Fraser Broth - Croatian - Croatia Fraser Broth - Czech - Czech Republic Fraser Broth - Danish - Denmark Fraser Broth - Dutch - Belgium Fraser Broth - Dutch - Netherlands Fraser Broth - English - Australia Fraser Broth - English - Canada Fraser Broth - English - EU/UK Fraser Broth - English - Ireland Fraser Broth - English - Malta Fraser Broth - English - New Zealand Fraser Broth - English - Philippines Fraser Broth - Estonian - Estonia Fraser Broth - Finnish - Finland Fraser Broth - French Fraser Broth - French - Belgium Fraser Broth - French - Canada Fraser Broth - German Fraser Broth - German - Austria Fraser Broth - German - Luxembourg Fraser Broth - Greek - Cyprus Fraser Broth - Greek - Greece Fraser Broth - Hungarian - Hungary Fraser Broth - Indonesian - Indonesia Fraser Broth - Italian - Italy Fraser Broth - Japanese - Japan Fraser Broth - Korean - Korea Fraser Broth - Latvian - Latvia Fraser Broth - Lithuanian - Lithuania Fraser Broth - Malay - Malaysia Fraser Broth - Maltese - Malta Fraser Broth - Norwegian - Norway Fraser Broth - Polish - Poland Fraser Broth - Portuguese - Brazil Fraser Broth - Portuguese - Portugal Fraser Broth - Romanian - Romania Fraser Broth - Slovak - Slovakia Fraser Broth - Slovene - Slovenia Fraser Broth - Spanish - Colombia Fraser Broth - Spanish - Mexico Fraser Broth - Spanish - Spain Fraser Broth - Spanish - US Fraser Broth - Swedish - Sweden Fraser Broth - Thai - Thailand Fraser Broth - Vietnamese - Vietnam

Technology

Lithium chloride suppresses the growth of Enterococci in the medium, as Enterococci can also hydrolyze esculin also. Enzymatic digest of animal tissues, casein, meat extract and yeast extract provide the essential vitamins, minerals, amino acids, nitrogen and carbon, while sodium chloride maintains the osmotic balance. The selective components acriflavine and nalidixic acid are blended into the base powder and inhibit the growth of Escherichia coli. Ferric ammonium citrate is added to the tempered broth after sterilization. According to ISO 11290-1:2017, the test portion is inoculated into the primary enrichment medium Half Fraser (Demi-Fraser) Broth (ISO), followed by secondary enrichment in Fraser Broth (ISO) and then sub-cultured onto Harlequin Listeria Chromogenic Agar according to Ottaviani & Agosti (NCM1004). The medium conforms to the performance and formulation requirements of ISO 11290-1:2017.