Fraser Broth Base

Item No.  NCM0066A

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Fraser Broth Base is used with Acriflavin, Nalidixic Acid, and Ferric Ammonium Citrate for the selective enrichment of Listeria spp. Fraser Broth Base is not intended for use in the diagnosis of disease or other conditions in humans.

Formulated according to MLG Appendix 1.09 (for the ISO 11290 formulation please use NCM0001). Listeria monocytogenes, described first in 1926 by Murray, Webb, and Swann, is an extensive problem in public health and food industries. This organism has the ability to cause human illness and death, particularly in immunocompromised individuals and pregnant women. Epidemiological evidence from outbreaks of listeriosis indicated the principle route of transmission is via the consumption of foodstuffs contaminated with Listeria monocytogenes. Implicated vehicles of transmission include turkey frankfurters, coleslaw, pasteurized milk, Mexican style cheese and pate.

Half Fraser (Demi Fraser) Broth Base is based on the formulation of Fraser and Sperber. This medium is used in rapid detection of Listeria from food and environmental samples. Listeria spp. grow over a pH range of 5.0 – 9.6, and survive in food products with pH levels outside these parameters. Listeria spp. are microaerophilic, Gram-positive, asporogenous, non-encapsulated, non-branching, short, motile rods. Motility is pronounced at 20°C. Identification of Listeria spp. is based on successful isolation of the organism, biochemical characterization, and serological confirmation.

Specifications
Analyte Listeria spp.
Brand NEOGEN® Culture Media
Formula
Formula Liter
Proteose Peptone 5.0 g/L
Tryptone 5.0 g/L
Beef Extract 5.0 g/L
Yeast Extract 5.0 g/L
Esculin 1.0 g/L
Disodium Hydrogen Phosphate 12.0 g/L
Potassium Dihydrogen Phosphate 1.35 g/L
Sodium Chloride 20.0 g/L
Lithium Chloride 3.0 g/L
Grams per Litre 57.40
Litres per 500g 9.00
Mass 500 g
Package Dimensions 4.60 IN x 2.90 IN x 8.60 IN
Package Weight 1.35 LB
Preparation
  1. Dissolve 57.4 grams of the medium in one liter of purified water.
  2. Mix thoroughly.
  3. Autoclave at 121°C for 15 minutes. Cool broth to room temperature.
  4. Aseptically add 2 vials of NCM4009-0.5* Ferric Ammonium Citrate and 10 mL of a filter sterilized solution containing 20 mg Nalidixic acid and 25 mg Acriflavin.

*Larger vials may be available. Please see appropriate supplement data sheet for availability and preparation instructions.

Previous Item Number Product was previously known as Acumedia Product: 7626 Fraser Broth Base

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