EC Medium with MUG

Item No.  NCM0107A

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EC Medium w/ MUG is used for the fluorogenic detection of Escherichia coli in a laboratory setting. EC Medium w/ MUG is not intended for use in the diagnosis of disease or other conditions in humans.

EC Medium was developed by Hajna and Perry in an effort to improve the methods for the detection of the coliform group and E. coli. This medium consists of a buffered lactose broth with the addition of 0.15% Bile Salts Mixture. Growth of spore-forming bacteria is inhibited by the bile salts, while growth of E. coli is enhanced by its presence. EC Medium w/ MUG is the same formula as EC Medium, with the addition of 4-methylumbelliferyl-ß-D-glucuronide. Feng and Hartman developed a rapid assay for E. coli by incorporating 4-methylumbelliferyl-ß-D-glucuronide (MUG) into Lauryl Tryptose Broth at a final concentration of 100 ɥg/mL. Moburg determined the amount of MUG could be reduced to a final concentration of 50 ɥg/mL without adversely affecting results.

EC Medium w/ MUG is prepared according to the formula specified by US EPA and methods for water and food testing.

Analyte E. coli
Brand NEOGEN® Culture Media
Formula Liter
Tryptose 20.0 g/L
Lactose 5.0 g/L
Bile Salts 1.5 g/L
Dipotassium Phosphate 4.0 g/L
Monopotassium Phosphate 1.5 g/L
Sodium Chloride 5.0 g/L
4-Methylumbelliferyl--D-Glucuronide 0.05 g/L
Grams per Litre 37.00
Litres per 500g 14.00
Mass 500 g
Package Dimensions 4.60 IN x 2.90 IN x 8.60 IN
Package Weight 1.35 LB
  1. Dissolve 37 g of the medium in one liter of purified water.
  2. Mix thoroughly.
  3. Distribute into tubes containing inverted Durham tubes.
  4. Autoclave at 121°C for 15 minutes.
Previous Item Number Product was previously known as Acumedia Product: 7361 EC Medium with MUG



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