Dichloran Glycerol (DG-18) Agar Base

Item No.  NCM0081A

This item has shipping restrictions.
In stock
Out of stock
Expect availability 01/28/2022
Minimum quantity required: 1
This item must be ordered in multiples of one case. Quantity per case: 1
Dichloran Glycerol (DG-18) Agar Base is used for the selective isolation and enumeration of yeasts and molds from foods in a laboratory setting. Dichloran Glycerol (DG-18) Agar Base is not intended for use in the diagnosis of disease or other conditions in humans. Dichloran Glycerol (DG-18) Agar Base is used for the enumeration of viable osmophilic yeasts and xerophilic molds in food or animal feed products with a water activity of less than or equal to 0.95. This includes such foods as dry fruits, jams, cakes, dried meat, salted fish, grains, cereals, flours, nuts, spices, condiments and some animal feeds. This medium is not suitable for the examination of dehydrated products with a water activity of greater than 0.95, Dichloran Rose Bengal Chloramphenicol Agar (NCM0082 or NCM0029) should be used. The reduction in water activity in this medium is achieved by the addition of glycerol at approximately 18% and this is very important as many yeast and molds require a low water activity to enhance growth and colony development. The medium also contains the antifungal agent dichloran, which restricts the spreading of mucoraceous fungi and restricts the colony size of other genera making colony counting an easier task. Additional selectivity against bacterial growth is achieved by the incorporation of the heat-stable antibiotic Chloramphenicol. Glucose is incorporated as the fermentable carbohydrate source, with casein enzymatic digest providing the essential vitamins, minerals, amino acids, nitrogen and carbon.  
Analyte Osmophilic Yeasts, Xerophilic Moulds
Brand NEOGEN® Culture Media
Formula Liter
Casein Enzymatic Digest 5.0 g/L
D-Glucose 10.0 g/L
Potassium Dihydrogen Phosphate 1.0 g/L
Magnesium Sulfate 0.5 g/L
Dichloran 0.002 g/L
Chloramphenicol 0.1 g/L
Agar 15.0 g/L
Grams per Litre 31.60
Litres per 500g 16.00
Mass 500 g
Package Dimensions 4.60 IN x 2.90 IN x 8.60 IN
Package Weight 1.35 LB
Plates per 500g 860.00
  1. Suspend 31.6 g of medium and 220 grams of glycerol in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.
  5. Cool to 45-50°C.
Previous Item Number Product was previously known as Acumedia Product: 7592 Dichloran Glycerol (DG-18) Agar Base; and LabM Product: LAB218 DG18 Agar - Dichloran (18%) Glycerol Agar



Our experts are here to help. If you need information beyond what is provided in Validation Reports under the Documents tab, please contact us and we will be happy to assist you. Learn more