Dermatophyte Test Medium (DTM)

Item No.  NCM0138A

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Expect availability 12/31/2050
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Dermatophyte Test Medium is used for the selective isolation of dermatophytic fungi and is not intended for use in the diagnosis of disease or other conditions in humans.

In 1969, Taplin et al. developed this medium for the isolation and recognition of dermatophytic fungi, the causative agent of ringworm from hair, nails, and skin. Dermatophyte Test Medium is preferred for isolation and early recognition of Microsporum, Trichophyton, and Epidermophyton genera because of a distinct color change in the medium. Rapidly-growing species may produce a complete color change in the medium in 3 days. The slower-growing species will change the indicator in longer time periods. Other organisms may grow but can be recognized as non-dermatophytes by lack of a color change. A few organisms, including saprophytes, yeasts, and bacteria are capable of changing the medium from red to yellow, but are easily recognized by their distinctive colonial morphology.

Specifications
Analyte Dermatophytic fungi
Brand NEOGEN® Culture Media
Formula
Formula Liter
Enzymatic Digest of Soybean Meal 10.0 g/L
Dextrose 10.0 g/L
Phenol Red 0.2 g/L
Cycloheximide 0.5 g/L
Agar 20.0 g/L
Grams per Litre 40.70
Litres per 500g 8.00
Mass 500 g
Package Dimensions 4.60 IN x 2.90 IN x 8.60 IN
Package Weight 1.35 LB
Plates per 500g 440.00
Preparation
  1. Suspend 40.7g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for once minute to completely dissolve medium.
  3. Autoclave at 121°C for 15 minutes.
  4. Cool to 45-50°C and aseptically add Gentamicin (0.1 g/L) and Chlortetracycline (0.1 g/L).
  5. Mix thoroughly.
Previous Item Number Product was previously known as Acumedia Product: 7265 Dermatophyte Test Medium (DTM); and LabM Product: LAB117 Dermatophyte Test Medium (DTM)

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