3M™ Demi Fraser Broth Base

Item No.  700002158  |  Catalog No.  BP0118500

  • 3M dehydrated media assist in keeping your lab practices.
  • Convenient: flexibility of options, make what you need when you need it.
  • Easy.
  • Consistent.
  • Cost effective.
This item has shipping restrictions.
$57.63 each
$248.56 each
In stock
Out of stock
Expect availability in 2-4 weeks
Minimum quantity required: 1
This item must be ordered in multiples of one case. Quantity per case: 1

Demi-Fraser Broth Base is used with ferric ammonium citrate for the selective enrichment of Listeria species and is not intended for use in the diagnosis of disease or other conditions in humans. The selective components acriflavine and nalidixic acid are blended into the base powder and the ferric ammonium citrate is added to the tempered broth after sterilization. Demi-Fraser Broth Base, is developed to give improved results for the isolation of Listeria spp. with both traditional and ELISA methods. As with the sister product, Fraser Broth Base, the selective components acriflavine and nalidixic acid are blended into the base powder and the Ferric Ammonium Citrate is added to the tempered broth after autoclaving. This format has been shown to give improved selectivity with pure cultures and food samples.

Furthermore, ELISA results are more stable, resulting in fewer false positive results when the ferric ammonium citrate is omitted from the complete media. Developed as a modification of UVM medium and made according to ISO 11290, Fraser Broth is a secondary enrichment broth for the isolation of Listeria spp. and is similar to Palcam Broth in that it contains esculin to indicate the presence of a potential Listeria isolate. It also contains lithium chloride in an attempt to suppress the growth of Enterococci in the medium (as does Palcam). Listeria spp. hydrolyze the esculin to form esculetin, which reacts with the ferric ammonium citrate in resulting in a black precipitate and a visible positive reaction. However, Enterococci can also perform this reaction so further plating is required onto an isolation medium such as Harlequin® Listeria Chromogenic Agar.

Mass 500 g
Quantity per Package 1 each
Package Weight 15.88 lb


Our customers’ success is our shared success. Our experts are ready to train you and your team on our solutions, so you can rest easy knowing procedures are performed properly and yield accurate results. In addition, we provide certificates upon completion of training to provide you with the documentation needed for audit traceability.