D/E Neutralizing Broth without Tween

Item No.  NCM0121A

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Minimum quantity required: 1
This item must be ordered in multiples of one case. Quantity per case: 1
D/E Neutralizing Broth is used to neutralize and determine the bactericidal activity of antiseptics and disinfectants. D/E Neutralizing Broth without Tween is not intended for use in the diagnosis of disease or other conditions in humans. D/E Neutralizing Broth was developed by Dey and Engley to neutralize a broad spectrum of disinfectants and preservative antimicrobial chemicals, including quaternary ammonium compounds, phenolics, iodine, chlorine preparations, mercurials, formaldehyde, and glutaraldehyde. D/E Neutralizing media neutralize higher concentrations of residual antimicrobials when compared with other standard neutralizing formulations, such as Letheen media, Thioglycollate media, and Neutralizing Buffer. Total neutralization of disinfectants is critical. Disinfectant residues can result in a false-negative (no-growth) test. D/E Neutralizing Broth without Tween effectively neutralizes the inhibitor action of disinfectant carryover, allowing differentiation between bacteriostasis and the true bactericidal action of disinfectant chemicals. This is a critical characteristic to consider when evaluating a disinfectant. D/E Neutralizing Broth without Tween is recommended for use in disinfectant evaluations, environmental sampling (swab and contact plate methods), and testing of water-miscible cosmetics.
Analyte Non-selective
Brand NEOGEN® Culture Media
Formula Liter
Enzymatic Digest of Casein 5.0 g/L
Yeast Extract 2.5 g/L
Dextrose (Glucose) 10.0 g/L
Sodium Thioglycollate 1.0 g/L
Sodium Thiosulfate 6.0 g/L
Sodium Bisulfite 2.5 g/L
Lecithin 7.0 g/L
Bromocresol Purple 0.02 g/L
Grams per Litre 34.00
Litres per 500g 13.00
Mass 500 g
Package Dimensions 4.60 IN x 2.90 IN x 8.60 IN
Package Weight 1.35 LB
  1. Dissolve 34 g of the medium and 5 grams of Tween (Polysorbate) 80 in one liter of purified water.
  2. Mix thoroughly.
  3. Autoclave at 121°C for 15 minutes.
Previous Item Number Product was previously known as Acumedia Product: 7562 DE Neutralizing Broth (no tween)

Additional Information

Supplement Polysorbate 80 (7992), 5g/L



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