Cystine Lactose Electrolyte Deficient (CLED) Agar

Item No.  NCM0034A

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Cystine Lactose Electrolyte Deficient (CLED) Agar is used for the differentiation and enumeration of microorganisms in urine. A medium for urine culture where the absence of electrolytes inhibits the swarming of Proteus spp. Cystine is added for the benefit of those organisms which have a specific cystine requirement. Differentiation of lactose and non-lactose fermenters is achieved using bromothymol blue as pH indicator. This medium supports the growth of Streptococcus pyogenes and most other fastidious organisms that do not require blood.  
Analyte Coliforms, E. coli, Enterobacteriaceae, Enterococci, Pseudomonas, Staphylococcus, Streptococci
Brand NEOGEN® Culture Media
Formula Liter
Balanced Peptone 4.0 g/L
Beef Extract 3.0 g/L
Tryptone 4.0 g/L
Lactose 10.0 g/L
L-Cystine 0.128 g/L
Bromothymol Blue 0.02 g/L
Agar 15.0 g/L
Grams per Litre 36.00
Litres per 500g 14.00
Mass 500 g
Package Dimensions 4.60 IN x 2.90 IN x 8.60 IN
Package Weight 1.35 LB
Plates per 500g 760.00
  1. Suspend 36 grams of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.
  4. Cool to 45-50°C.
Previous Item Number Product was previously known as Acumedia Product: 7122 CLED Agar; and LabM Product: LAB041 CLED Medium (Mackey and Sandys)



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