Columbia CNA Agar
SKU No.  700003296  | Catalog No.  NCM0115A
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Columbia CNA Agar

SKU No.  700003296  |  Catalog No.  NCM0115A

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Columbia CNA Agar is used with blood for the selective isolation of Gram-positive cocci in a laboratory setting. Columbia CNA Agar is not intended for use in the diagnosis of disease or other conditions in humans.

Ellner et al. described Columbia CNA Agar as a variation of Columbia Blood Agar Base that is selective for Gram-positive cocci. Colistin and Nalidixic Acid are added to the formula, selecting for Gram-positive organisms and fungi by suppressing Gram-negative bacteria.

Specifications
BrandNeogen® Culture Media
AnalyteGram-positive cocci
PlatformDehydrated Culture Media
Mass500 g
Formula
FormulaLiter
Enzymatic Digest of Casein5.0 g/L
Enzymatic Digest of Animal Tissue8.0 g/L
Enriched Peptone10.0 g/L
Starch1.0 g/L
Sodium Chloride5.0 g/L
Colistin0.015 g/L
Nalidixic Acid0.01 g/L
Agar14.0 g/L
Grams per Liter43.00
Liters per 500 g12.00
Plates per 500 g630.00
Preparation
  1. Suspend 43 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes. Do not overheat medium.
  4. Cool to 45 - 50°C.
  5. Prepare 5% blood agar by aseptically adding the appropriate volume of sterile defibrinated blood to melted sterile agar medium.
Previous Item NumberProduct was previously known as Acumedia Product: 7126 Columbia CNA Agar
Package Dimensions4.60 in x 2.90 in x 8.60 in
Package Weight1.35 lb

Documents

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Columbia CNA Agar - Technical Specifications Sheet - English - US

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