Colitag™ Water Test System

Item No.  9850

  • A presence/absence or most probable number test for total coliforms and E. coli in water
  • Offers results in 16–48 hours
  • Detects 1 CFU of total coliforms or E. coli
This item has shipping restrictions.
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Expect availability in 2-4 weeks
Minimum quantity required: 1
This item must be ordered in multiples of one case. Quantity per case: 1

Colitag™ is a selective and differential ready-to-use, dehydrated medium for the simultaneous detection of total coliform bacteria and Escherichia coli in water.

Colitag is approved by the U.S. Environmental Protection Agency for compliance sample analyses as required by the Revised Total Coliform Rule. This method can be used as a presence/absence (P/A) test or for quantification of bacteria through several most probable number (MPN) formats.

Colitag is capable of detecting 1 CFU of total coliforms or E. coli in a 100 mL water sample in 16–48 hours and does not require further confirmation or verification steps.

Analyte Coliforms, E. coli
Application Potable Water Applications
  • EPA approved for determination of total coliforms and E. coli in drinking water at 40 CFR 141.852(a)(5)
    1. EPA: Federal Register/Vol. 86, No. 100/ May 2021/Rules and Regulations
    2. EPA: Federal Register/Vol. 74, No. 216/Tuesday, November 10, 2009/Rules and Regulations
  • EPA approved for determining E. coliunder the Ground Water Rule.
    1. Analytical Methods Approved for Drinking Water Compliance Monitoring Under the Ground Water Rule (
  • FDA approved for dairy source waters.
    1. NCIMS Form 2400-M Revision July 2020: Chromogenic substrate (MMO-MUG) presence-absence test for total coliform and E. coli in dairy source waters
    2. NCIMS M-A-98 Revision April 2020: Dairy water — Individual water supply system (private well, spring, surface water, hauled water), reclaimed water, pasteurized equivalent water
  • FDA inclusion in agricultural water.
    1. Standards for the Growing, Harvesting, Packing, and Holding of Produce for Human Consumption
    2. FDA fact sheet
Brand Colitag™
Incubation Time 16 - 48 Hours
Limit of Detection 1 CFU in a 100 mL sample
Matrix Water
Package Dimensions 4.40 IN x 4.00 IN x 2.60 IN
Package Weight 0.10 LB
Platform Dehydrated Culture Media
Quantity per Package 20 tests
Result Type Presence/Absence, Qualitative
Storage Conditions 2 - 30°C (4 - 7°C preferred)
Testing Time 16.00 - 48.00 Hours

Colitag test kits, 100 mL format
  1. Incubator set to 35°C ± 0.5°
  2. Long wavelength (365 nanometer) UV lamp
  3. Sterile sample containers large enough to contain 100 mL water samples
  4. UV protective safety glasses


  1. Colitag Comparator
  2. Circulating, covered water bath capable of maintaining 35°C or 44.5°C for prewarming samples
  3. Circulating, covered water bath capable of maintaining 35°C or 44.5°C for prewarming samples. Control bacteria strains (see recommended quality control strains in product insert)


Our customers’ success is our shared success. Our experts are ready to train you and your team on our solutions, so you can rest easy knowing procedures are performed properly and yield accurate results. In addition, we provide certificates upon completion of training to provide you with the documentation needed for audit traceability.



Our experts are here to help. If you need information beyond what is provided in Validation Reports under the Documents tab, please contact us and we will be happy to assist you. Learn more