CLED Medium (Bevis Modification)

Item No.  NCM0220A

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CLED Medium (Bevis Modification) is for the cultivation of coliforms, staphylococci and streptococci, and is not intended for use in the diagnosis of disease or other conditions in humans.

Bevis modified Mackey and Sandys original medium by introducing a double indicator to improve the differentiation of lactose and non-lactose fermenting coliforms, staphylococci and streptococci. The swarming of Proteus spp. is inhibited. Neogen’s NCM0220 CLED Medium will grow many of the more demanding streptococci of Lancefield groups A, B, C, G and F. This medium may not grow Pasteurella spp. or halophilic organisms.

Analyte Coliforms, Staphylococci, Streptococci
Brand NEOGEN® Culture Media
Formula Liter
Balanced Peptone 4.0 g/L
Beef Extract 3.0 g/L
Tryptone 4.0 g/L
Lactose 10.0 g/L
L-Cystine 0.128 g/L
Bromothymol Blue Indicator 0.02 g/L
Andrade’s Indicator 0.08 g/L
Agar 15.0 g/L
Grams per Litre 36.00
Litres per 500g 14.00
Mass 500 g
Package Dimensions 4.60 IN x 2.90 IN x 8.60 IN
Package Weight 1.35 LB
Plates per 500g 760.00
  1. Suspend 36 grams of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.
  4. Cool to 45-50°C.
Previous Item Number Product was previously known as LabM Product: LAB006 CLED Medium (Bevis)



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