Campy Blood-Free Selective Medium (Modified CCDA) (EMEA)

Item No.  NCM0195A

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Campylobacter Blood-Free Selective Medium (Modified Charcoal Cefoperazone Deoycholate Agar or mCCDA) is used for the cultivation of campylobacters, and is not intended for use in the diagnosis of disease or other conditions in humans.

A blood free medium which will support the growth of most enteric campylobacters. The selective supplement X112 (X212) makes the medium selective for C. jejuni. and C. coli when incubated at 37°C. With this product incubation at 42°C is no longer necessary and higher recovery rates have been reported at 37°C than at 42°C. The supplement X112 (X212) consists of cefoperazone and amphotericin and is superior to the selective combination of Skirrow, Butzler and Blazer-Wang all of which contain antibiotics shown to be inhibitors to C. coli. The colonial morphologies of Campylobacter spp. on this medium are distinctive.

Specifications
Analyte Campylobacter spp.
Brand NEOGEN® Culture Media
Formula
Formula Liter
Peptone Mix 25.0 g/L
Bacteriological Charcoal 4.0 g/L
Sodium chloride 3.0 g/L
Sodium Deoxycholate 1.0 g/L
Ferrous Sulphate 0.25 g/L
Sodium Pyruvate 0.25 g/L
Agar 12.0 g/L
Grams per Litre 45.00
Litres per 500g 11.00
Mass 500 g
Package Dimensions 4.60 IN x 2.90 IN x 8.60 IN
Package Weight 1.35 LB
Plates per 500g 610.00
Preparation
  1. Suspend 45.5 grams of the medium and add to 1 liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.
  4. Cool to 45-50°C then add 2 vials of X112 supplement, mix well and pour into Petri dishes.
  5. Continuously mix whilst pouring to prevent the charcoal settling.
Previous Item Number Product was previously known as LabM Product: LAB112 Campylobacter Blood-Free Selective Medium (Modified CCDA) (EMEA)

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