Burkholderia Cepacia Selective Agar (BCSA), Complete

Item No.  NCM0209A

Burkholderia Cepacia Selective Agar, Complete is used for the selective isolation of the Burkholderia (Pseudomonas) cepacia Complex (Bcc). Burkholdaria Cepacia Selective Agar is not intended for use in the diagnosis of disease or other conditions in humans.

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Minimum quantity required: 1
This item must be ordered in multiples of one case. Quantity per case: 1

The United States Pharmacopeia has updated the USP/NF Microbiological Test <60> Microbiological Examination of Nonsterile Products- Tests for Burkholderia cepacia Complex (Bcc) to include the use of this medium. This test is designed to determine whether a substance or preparation complies with an established specification for microbiological quality and/or to evaluate whether product contains members of the Bcc.

Burkholderia Cepacia Selective Agar, Complete is the complete medium formulation specified by the USP/NF. BCSA, Complete contains peptones and sugars that supply nutrients for the growth of the Bcc. Phenol Red is the sugar utilization indicator. Crystal Violet is added to inhibit the growth of Gram-positive bacteria. Antimicrobics are added to suppress or inhibit the recovery bacteria other than the Bcc. Agar is the solidifying agent.

Analyte Burkholderia Cepacia
Brand NEOGEN® Culture Media
Formula Liter
Enzymatic Digest of Casein 10.0 g/L
Lactose 10.0 g/L
Sucrose 10.0 g/L
Sodium Chloride 5.0 g/L
Yeast Extract 1.5 g/L
Phenol Red 0.08 g/L
Crystal Violet 0.002 g/L
Vancomycin 0.0025 g/L
Gentamicin 0.01 g/L
Polymyxin B 600,000 IU/L
Agar 14.0 g/L
Grams per Litre 50.69
Litres per 500g 9.90
Mass 500 g
Package Dimensions 4.60 IN x 2.90 IN x 8.60 IN
Package Weight 1.35 LB
Plates per 500g 657.60
Platform Dehydrated Culture Media
  1. Suspend 50.69 grams of the medium in one liter of purified water.
  2. Heat with frequent agitation and bring to a boil. Avoid overheating.
  3. Autoclave at 121°C for 15 minutes.
  4. Cool to 45-50°C.
  5. Avoid holding for longer than the time required to equilibrate.
Quantity per Package 1 bottle
Storage Conditions 2-8°C


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