Buffered Peptone Water HQ (ISO)

Item No.  NCM0270A

Buffered Peptone Water HQ (ISO) is a non-selective pre-enrichment medium for the isolation of Salmonella spp. in food, animal feed and in environmental samples from the food production area as described in ISO 6579-1:2017. It can be used with Salmonella Selective Supplement (NCM4000) as part of the OBOP-S alternative workflow to ISO 6579-1:2017.

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Minimum quantity required: 1
This item must be ordered in multiples of one case. Quantity per case: 1

This pre-enrichment medium is designed to aid resuscitation of sub-lethally damaged Salmonella before introduction into a selective medium. Sublethal injury to Salmonella occurs in many food processes and this pre-enrichment step greatly increases recovery of these organisms. Buffered Peptone Water HQ (ISO) can be used in conjunction with Salmonella Selective Supplement (NCM4000) as part of the OBOP-S alternative workflow to ISO 6579-1:2017. Salmonella Selective Supplement is provided separately to enable other analysis (i.e. enumeration tests) prior to selective enrichment for Salmonella spp.

Buffered Peptone Water HQ (ISO) follows the ISO formulation. Peptone provides a source of carbon, amino acids, vitamins and minerals. Sodium chloride maintains the osmotic balance and the phosphate components buffer the media to achieve pH 7.0.

Analyte Salmonella spp.

This product forms part of NEOGEN’s innovative One Broth One Plate for Salmonella (OBOP-S) workflow that has been validated according to ISO 16140-2 and certified by both MicroVal and AOAC.

Brand NEOGEN® Culture Media
Formula Liter
Peptone 10.0 g/L
Sodium Chloride 5.0 g/L
Disodium Hydrogen Phosphate (anhydrous) 3.6 g/L
Potassium Dihydrogen Phosphate 1.5 g/L
Grams per Litre 20.10
Litres per 500g 24.90
Mass 500 g
Platform Dehydrated Culture Media, One Broth, One Plate
  1. Dissolve 20.1 g of the medium in one liter of purified water.
  2. Mix thoroughly.
  3. Autoclave at 121°C for 15 minutes.
  4. If used for selective enrichment as part of the alternative workflow, add 1 mL of reconstituted Salmonella Selective Supplement (NCM4000) to 225 mL of prepared base.
Quantity per Package 1 bottle
Result Type Presence/Absence
Storage Conditions 2-30 °C away from direct sunlight

Additional Information

Buffered Peptone Water HQ (ISO) forms part of NEOGEN's innovative One Broth One Plate for Salmonella (OBOP-S) workflow. The OBOP-S workflow has the following external validations and certifications: AOAC RI Validation PTM 102002 and MicroVal Validation Certification 2019LR88


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