Buffered Peptone Water HQ (ISO)

Item No.  NCM0270A

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Buffered Peptone Water HQ (ISO) is a non-selective pre-enrichment medium for isolating Salmonella spp. in food, animal feed, and environmental samples from the food production area as described in ISO 6579-1:2017. It can be used with Salmonella Selective Supplement (NCM4000) as part of Neogen's One Broth One Plate for Salmonella alternative method workflow.

This pre-enrichment medium is designed to aid resuscitation of sub-lethally damaged Salmonella before introduction into a selective medium. Sublethal injury to Salmonella occurs in many food processes, and this pre-enrichment step greatly increases the recovery of these organisms. Salmonella Selective Supplement is provided separately to enable other analysis (i.e., enumeration tests) prior to selective enrichment for Salmonella spp.

Buffered Peptone Water HQ (ISO) follows the ISO formulation. Peptone provides a source of carbon, amino acids, vitamins, and minerals. Sodium chloride maintains the osmotic balance, and the phosphate components buffer the media to achieve pH 7.0.

Specifications
Brand Neogen® Culture Media
Analyte Salmonella spp.
Platform Dehydrated Culture Media, One Broth One Plate
Result Type Presence/Absence
Approvals This product forms part of Neogen's innovative One Broth One Plate for Salmonella (OBOP-S) workflow that has been validated according to ISO 16140-2 and certified by both MicroVal and AOAC.
Mass 500 g
Formula
Formula Liter
Peptone 10.0 g/L
Sodium Chloride 5.0 g/L
Disodium Hydrogen Phosphate (anhydrous) 3.6 g/L
Potassium Dihydrogen Phosphate 1.5 g/L
Grams per Liter 20.10
Liters per 500 g 24.90
Preparation
  1. Dissolve 20.1 g of the medium in one liter of purified water.
  2. Mix thoroughly.
  3. Autoclave at 121°C for 15 minutes.
  4. If used for selective enrichment as part of the alternative workflow, add 1 mL of reconstituted Salmonella Selective Supplement (NCM4000) to 225 mL of prepared base.
Storage Conditions 2-30 °C away from direct sunlight
Quantity per Package 1 bottle

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