Buffered Listeria Enrichment Broth

Item No.  NCM0051A

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Buffered Listeria Enrichment Broth is used for selective enrichment of Listeria spp and is not intended for use in the diagnosis of disease or other conditions in humans.

Listeria monocytogenes, described first in 1926 by Murray, Webb, and Swann, is an extensive problem in public health and food industries. Evidence from outbreaks of listeriosis has indicated that the principle route of transmission is via consumption of foodstuffs contaminated with Listeria monocytogenes. Implicated vehicles of transmission include turkey, frankfurters, coleslaw, pasteurized milk, Mexican style cheese, and pate. Listeria spp. are ubiquitous in nature, being present in a wide range of unprocessed foods as well as in soil, sewage, and river water.

Buffered Listeria Enrichment Broth, a modification of the formula by Lovett et al., was developed after subsequent work concluded that enrichment properties can be improved by increasing the buffering capacity of the medium with the addition of Disodium Phosphate. Listeria spp. grow over a pH range of 5.0 - 9.6, and survive in food products with pH levels outside these parameters. Listeria spp. are microaerophilic, Gram-positive, asporogenous, non-encapsulated, non-branching, short, motile rods. Motility is pronounced at 20°C. Identification of Listeria is based on successful isolation of the organism, biochemical characterization, and serological confirmation.

Specifications
Brand Neogen® Culture Media
Analyte Listeria spp.
Platform Dehydrated Culture Media
Mass 500 g
Formula
Formula Liter
Enzymatic Digest of Casein 17.0 g/L
Enzymatic Digest of Soybean Meal 3.0 g/L
Yeast Extract 6.0 g/L
Dextrose 2.5 g/L
Sodium Chloride 5.0 g/L
Monpotassium Phosphate 1.35 g/L
Dipotassium Phosphate 2.5 g/L
Disodium Phosphate 9.6 g/L
Cycloheximide 0.05 g/L
Nalidixic Acid 0.04 g/L
Acriflavin 0.01 g/L
Grams per Liter 47.00
Liters per 500 g 11.00
Preparation
  1. Dissolve 47 g of the medium in one liter of purified water.
  2. Mix thoroughly.
  3. Autoclave at 121°C for 15 minutes.
Previous Item Number Product was previously known as Acumedia Product: 7579 Buffered Listeria Enrichment Broth; and LabM Product: LAB579 Buffered Listeria Enrichment Broth
Package Weight 1.35 lb

Documents

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Buffered Listeria Enrichment Broth - English - US Buffered Listeria Enrichment Broth - English - US Buffered Listeria Enrichment Broth - Deutsch Buffered Listeria Enrichment Broth - Deutsch Buffered Listeria Enrichment Broth - English - Great Britain Buffered Listeria Enrichment Broth - English - Great Britain Buffered Listeria Enrichment Broth - Français Buffered Listeria Enrichment Broth - Français NCM0051_7579_SDS_da-DK.pdf NCM0051_7579_SDS_en-AU.pdf NCM0051_7579_SDS_en-CA.pdf NCM0051_7579_SDS_en-NZ.pdf NCM0051_7579_SDS_en-PH.pdf NCM0051_7579_SDS_es-005.pdf NCM0051_7579_SDS_es-419.pdf NCM0051_7579_SDS_es-US.pdf NCM0051_7579_SDS_fi-FI.pdf NCM0051_7579_SDS_fr-CA.pdf NCM0051_7579_SDS_hu-HU.pdf NCM0051_7579_SDS_id-ID.pdf NCM0051_7579_SDS_it-IT.pdf NCM0051_7579_SDS_ja-JA.pdf NCM0051_7579_SDS_ko.pdf NCM0051_7579_SDS_ms-MY.pdf NCM0051_7579_SDS_nl-NL.pdf NCM0051_7579_SDS_no-NO.pdf NCM0051_7579_SDS_pl-PL.pdf NCM0051_7579_SDS_pt-BR.pdf NCM0051_7579_SDS_sv-SE.pdf NCM0051_7579_SDS_th-TH.pdf NCM0051_7579_SDS_vi-VN.pdf NCM0051_7579_SDS_zh-Hans-CN.pdf NCM0051_7579_SDS_zh-Hant-TW.pdf NCM0051_LAB579_7579_SDS_da-DK.pdf NCM0051_LAB579_7579_SDS_en-AU.pdf NCM0051_LAB579_7579_SDS_en-CA.pdf NCM0051_LAB579_7579_SDS_en-NZ.pdf NCM0051_LAB579_7579_SDS_en-PH.pdf NCM0051_LAB579_7579_SDS_es-005.pdf NCM0051_LAB579_7579_SDS_es-419.pdf NCM0051_LAB579_7579_SDS_es-US.pdf NCM0051_LAB579_7579_SDS_fi-FI.pdf NCM0051_LAB579_7579_SDS_fr-CA.pdf NCM0051_LAB579_7579_SDS_hu-HU.pdf NCM0051_LAB579_7579_SDS_id-ID.pdf NCM0051_LAB579_7579_SDS_it-IT.pdf NCM0051_LAB579_7579_SDS_ja-JA.pdf NCM0051_LAB579_7579_SDS_ko.pdf NCM0051_LAB579_7579_SDS_ms-MY.pdf NCM0051_LAB579_7579_SDS_nl-NL.pdf NCM0051_LAB579_7579_SDS_pt-BR.pdf NCM0051_LAB579_7579_SDS_sv-SE.pdf NCM0051_LAB579_7579_SDS_vi-VN.pdf NCM0051_LAB579_7579_SDS_zh-Hans-CN.pdf NCM0051_LAB579_7579_SDS_zh-Hant-TW.pdf

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