Buffered Listeria Enrichment Broth Base

Item No.  NCM0164A

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Buffered Listeria Enrichment Broth Base is used with supplements for selective enrichment of Listeria spp. in foods and is not intended for use in the diagnosis of human disease.

Listeria monocytogenes, described first in 1926 by Murray, Webb, and Swann, is an extensive problem in public health and food industries. This organism has the ability to cause human illness and death, particularly in immunocompromised individuals and pregnant women. Epidemiological evidence from outbreaks of listeriosis has indicated that the principle route of transmission is via consumption of foodstuffs contaminated with Listeria monocytogenes. Implicated vehicles of transmission include turkey, frankfurters, coleslaw, pasteurized milk, Mexican style cheese, and pate.

Buffered Listeria Enrichment Broth, a modification of the formula by Lovett et al. was developed after research concluded that enrichment properties can be improved by increasing the buffering capacity of the medium with the addition of disodium phosphate. BLEB Base is based upon the FDA/BAM recommendations where the medium is supplemented with selective agents after an initial four hour, non-selective, pre-enrichment.

Analyte Listeria spp.
Brand NEOGEN® Culture Media
Formula Liter
Enzymatic Digest of Casein 17.0 g/L
Enzymatic Digest of Soybean Meal 3.0 g/L
Yeast Extract 6.0 g/L
Dextrose 2.5 g/L
Sodium Chloride 5.0 g/L
Monopotassium Phosphate 1.35 g/L
Dipotassium Phosphate 2.5 g/L
Disodium Phosphate 9.6 g/L
Grams per Litre 47.00
Litres per 500g 11.00
Mass 500 g
Package Dimensions 4.60 IN x 2.90 IN x 8.60 IN
Package Weight 1.35 LB
  1. Dissolve 47 g of the medium in 1000 mL of purified water.
  2. Mix thoroughly.
  3. Autoclave at 121°C for 15 minutes.
  4. Aseptically add 11.1 mL of a 10% filter sterilized solution of sodium pyruvate.
  5. After four hours of incubation at 30 ± 2°C, aseptically add 2.5 mL Buffered Listeria Enrichment Supplement (7980) to 225 mL of Buffered Listeria Enrichment Broth Base containing 25 grams of the sample. OR, aseptically add 0.455 mL of a 0.5% aqueous solution of acriflavine, 1.8 mL of a 0.5% aqueous solution of nalidixic acid, and 1.15 mL of a 1.0% solution of cycloheximide in 40% ethanol to 225 mL of medium containing 25 g or 25 mL of food to be tested.
Previous Item Number Product was previously known as Acumedia Product: 7675 Buffered Listeria Enrichment Broth Base (BLEB); and LabM Product: LAB139/LAB582 Buffered Listeria Broth



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