Brilliant Green Agar (Modified)

Item No.  NCM0058A

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Brilliant Green Agar is used for the selection and differentiation of Salmonella (other than S. typhi) from foods or feedstuffs. Brilliant Green Agar is not intended for use in the diagnosis of disease or other conditions in humans. First introduced by Kristensen et al in 1925 as a selective medium for the isolation of Salmonella (except S. typhi). The medium was modified by the Netherlands Institute for Public Health, Utrecht. The modification was to increase the selectivity of the medium by increasing the dye concentration. This formulation is quoted by the International Standards Organization, standard European Community Methods, the American Public Health Association and the Association of Official Analytical Chemists. The medium is suitable for subcultures from selective enrichment media. However, because this medium is highly selective, small numbers of salmonellae may be missed. This medium is not recommended for S. typhi and Shigella spp. Less inhibitory media such as X.L.D. (NCM0021) and Hektoen Enteric Agar (NCM0006) will be useful in detecting Salmonella typhi and Shigella spp. which are inhibited by Brilliant Green Agar.  
Analyte Salmonella spp.
Brand NEOGEN® Culture Media
Formula Liter
Beef Extract 5.0 g/L
Balanced Peptone 10.0 g/L
Yeast Extract 3.0 g/L
Disodium Hydrogen Phosphate 1.0 g/L
Sodium Dihydrogen Phosphate 0.6 g/L
Lactose 10.0 g/L
Sucrose 10.0 g/L
Phenol Red 0.09 g/L
Brilliant Green 0.0047 g/L
Agar 12.0 g/L
Grams per Litre 52.00
Litres per 500g 10.00
Mass 500 g
Package Dimensions 4.60 IN x 2.90 IN x 8.60 IN
Package Weight 1.35 LB
Plates per 500g 520.00
  1. Suspend 52 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Cool to 45-50°C.
Previous Item Number Product was previously known as Acumedia Product: 9212 Brilliant Green Agar; and LabM Product: LAB034 Brilliant Green Agar (Modified)