BCYE Agar (Legionella Isolation Medium)

Item No.  NCM0037A

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Minimum quantity required: 1
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BCYE Agar is used (with appropriate supplementation) for the isolation of Legionella spp. from water and environmental samples. BCYE Agar is not intended for use in the diagnosis of disease or other conditions in humans.

In 1977, McDade et al. identified Legionella pneumophila as the causative agent of Legionnaires’ disease, a multisystem disease manifested primarily by pneumonia. In 1978 a new medium, F-G Agar, resulted in improved growth of L. pneumophila, a very fastidious organism. Freely et al. modified F-C Agar by substituting yeast extract as a vitamin source and replacing starch with activated charcoal, producing Charcoal Yeast Extract (CYE) Agar. In 1980, Pasculle et al. reported that CYE Agar could be improved by the addition of ACES (N-2-acetamido-2-aminoethane sulfonic acid) buffer. One year later, Edelstein further increased the sensitivity of the medium by adding the potassium salt of alpha-ketoglutaric acid.

Brand Neogen® Culture Media
Analyte Legionella spp.
Platform Dehydrated Culture Media
Mass 500 g
Formula Liter
Yeast Extract 10.0 g/L
Charcoal 2.0 g/L
Ferric Pyrophosphate 0.25 g/L
ACES Buffer 10.0 g/L
Potassium Carbonate 2.3 g/L
Agar 14.0 g/L
Grams per Liter 38.50
Liters per 500 g 13.00
Plates per 500 g 710.00
  1. Suspend 38.5 grams of the medium in 1 L of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 110°C for 10 minutes. Cool to 45 - 50°C.
  4. Aseptically add 2 vials of NCM4006-0.5* BCYE Growth Supplement, each reconstituted using 5 mL sterile deionized/RO water, to make BCYE agar with Legionella growth supplements.
  5. To make GVPC selective medium additionally add 2 vials of NCM4007-0.5* GVPC Selective Supplement, each reconstituted using 10 mL sterile deionized/RO water.
  6. Mix thoroughly and continuing mixing throughout dispensing. NOTE: NCM4006 supplement can be substituted with NCM4005 supplement (without the addition of NCM4007 selective supplement) to prepare presumptive identification plates.

*Larger vials may be available. Please see appropriate supplement data sheet for availability and preparation instructions.

Previous Item Number Product was previously known as Acumedia Product: 7728 BCYE Agar Base; and LabM Product: LAB195 BCYE Legionella Isolation Medium
Package Weight 1.35 lb



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