Bacillus cereus Agar Base (PEMBA)

Item No.  NCM0165A

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Minimum quantity required: 1
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Bacillus Cereus Agar Base is used with Polymyxin B and Egg Yolk Emulsion for the isolation and presumptive identification of Bacillus cereus and is not intended for use in the diagnosis of human disease.

Holbrook and Anderson described a highly selective and diagnostic medium (PEMBA) for the isolation and determination of Bacillus cereus from food. The medium is formulated to detect small numbers of B. cereus in the presence of large number of contaminants. This medium differentiates B. cereus from other bacteria based on resistance to polymyxin, lack of mannitol fermentation, and presence of lecithinase. B. cereus can be found on vegetables, processed foods, and in nature. B. cereus causes gastrointestinal illness if the organism is allowed to proliferate. Outbreaks of foodborne illness have been associated with boiled and cooked rice, cooked meats, and cooked vegetables.

Specifications
Analyte Bacillus cereus
Brand NEOGEN® Culture Media
Formula
Formula Liter
Enzymatic Digest of Casein 1.0 g/L
Mannitol 10.0 g/L
Sodium Chloride 2.0 g/L
Magnesium Sulfate 0.1 g/L
Disodium Phosphate 2.5 g/L
Monopotassium Phosphate 0.25 g/L
Bromothymol Blue 0.12 g/L
Sodium Pyruvate 10.0 g/L
Agar 15.0 g/L
Grams per Litre 41.00
Litres per 500g 12.00
Mass 500 g
Package Dimensions 4.60 IN x 2.90 IN x 8.60 IN
Package Weight 1.35 LB
Plates per 500g 670.00
Preparation
  1. Suspend 41 grams of the medium in 965 mL of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.
  4. Cool to 45 - 50°C. Aseptically add 25 mL of NCM4017* Egg Yolk Emulsion 50% and 2 vials of NCM4032-0.5* Polymyxin B reconstituted using 5 mL sterile deionized/RO water.

*Larger vials may be available. Please see appropriate supplement data sheet for availability and preparation instructions.

Previous Item Number Product was previously known as Acumedia Product: 7442 Bacillus Cereus Agar Base; and LabM Product: LAB193 PEMBA (Bacillus Cereus Medium)

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