ANSR® for Listeria

1. 12 strips of 8 cluster tubes, 1.2 mL
2. 12 strips of 8 reaction tubes, 200 µL, containing lyophilized ANSR for Listeria reagents in 2 sealed foil pouches with desiccant pack
3. 12 strips of 8 permanent caps for reaction tubes
4. 1 bottle lysis reagent suspension buffer, 60 mL
5. 3 vials containing lyophilized lysis reagents
6. 1 kit insert

Equipment Required

1. ANSR reader (Neogen item 9828)
2. Computer and software for connection to ANSR reader (Neogen item 9832)
3. 1 dual heater block with lysis block inserts for 1.2 mL cluster tubes, 80 ± 2°C and 37 ± 2°C (Neogen items 9386-DUAL48, 9829-48)
4. Pipettor, 100 – 1000 µL (Neogen item 9463)
5. Pipette tip rack, 100 – 1000 µL, sterile, 96 tips (Neogen item 9487)
6. Pipettor, 10 – 100 µL, 8-channel (Neogen item 9388)
7. Pipette tips, 100 µL, sterile, filtered, 96 tips (Neogen item 9389)
8. Vortex mixer, adjustable speed (Neogen item 9494)
9. Stomacher (optional)
10. 3 thermometers, traceable (Neogen item 9518)
11. Timer, 3-channel (Neogen item 9426)
12. Optional heater block with block insert for 0.2 mL reaction tubes, 56 ± 1°C (Neogen items 9386-48D, 9829-64)
13. Webcam (Neogen item WEBCAM)
14. ANSR ethernet cable (Neogen item 9835)
15. Pipette pump, 10 mL (Neogen item 9277)
16. Pippettes, sterile serological, 10 mL (Neogen item 8686)
17. 40-slot, 20 mm test tube rack, autoclavable (Neogen item 9553)
18. Pipettor, 20-200 µL (Neogen item 9276)

Other Materials Required

1. Stomacher-type bags for sample enrichment. Filtered bags are recommended (Neogen item 6827)
2. Graduated cylinder, 250 mL (Neogen item 9368)

Media Enrichment Broth Required

1. ANSR for Listeria Enrichment Broth 1 (Neogen item 9814) - for environmental samples
2. LESS Medium, 500 g bottle (Neogen item 9790A) - for food samples except meats
3. Reveal^® Buffered Listeria Enrichment Broth (BLEB), 500 g bottle (Neogen item 9786) - for meat samples. Also used for confirmation, see page 6 of Kit Insert.
4. LESS Plus Medium, 500 g bottle (Neogen item 9880) - for select matrices.
5. 1 L purified water
6. Phosphate Buffered Saline (if testing smoked salmon or guacamole) (Neogen item 8425)