ANSR® for E. coli O157:H7

1. 12 strips of 8 cluster tubes, 1.2 mL
2. 12 strips of 8 reaction tubes, 200 µL, containing lyophilized ANSR for E. coli O157:H7 reagents in 2 sealed foil pouches with desiccant pack
3. 12 strips of 8 permanent caps for reaction tubes
4. 1 bottle lysis reagent suspension buffer, 60 mL
5. 3 vials containing lyophilized lysis reagents
6. 1 kit insert

Equipment Required

1. ANSR reader (Neogen item 9828)
2. Computer and software for connection to ANSR reader (Neogen item 9832)
3. 1 dual heater block with lysis block inserts for 1.2 mL cluster tubes, 80 ± 2°C and 37 ± 2°C (Neogen items 9386-DUAL48, 9829-48)
4. Pipettor, 100 – 1000 µL (Neogen item 9463)
5. Pipette tip rack, 100 – 1000 µL, sterile, 96 tips (Neogen item 9487)
6. Pipettor, 10 – 100 µL, 8-channel (Neogen item 9388)
7. Pipette tips, 100 µL, sterile, filtered, 96 tips (Neogen item 9389)
8. Vortex, adjustable speed (Neogen item 9494)
9. Stomacher (optional)
10. 3 thermometers, traceable (Neogen item 9518)
11. Timer, 3-channel (Neogen item 9426)
12. Optional heater block with block insert for 0.2 mL reaction tubes, 56 ± 1°C (Neogen items 9386-48D, 9829-64)
13. Webcam (Neogen item WEBCAM)
14. ANSR ethernet cable (Neogen item 9835)
15. Pipette pump, 10 mL (Neogen item 9277)
16. Pippettes, sterile serological, 10 mL (Neogen item 8686)
17. 40-slot, 20 mm test tube rack, autoclavable (Neogen item 9553)
18. Pipettor, 20-200 µL (Neogen item 9276)

Other Materials Required

1. Stomacher-type bags for sample enrichment. Filtered bags are recommended (Neogen item 6833)
2. Graduated cylinder, 250 mL (Neogen item 9368)

Media Enrichment Broth Required

1. ANSR for E. coli Enrichment Broth (Neogen item 9815)
2. Tryptic Soy Broth, Modified with Acid Digest of Casein (mTSB) (Neogen item 7167A)
3. 1 L purified water
4. Phosphate Buffered Saline (Neogen item 8425)