Acutone PPLO Broth without Crystal Violet

Item No.  NCM0053A

This item has shipping restrictions.
$83.50
$789.00
$1,543.00
$7,542.00
In stock
Out of stock
Expect availability in 2-4 weeks
Qty:
Minimum quantity required: 1
This item must be ordered in multiples of one case. Quantity per case: 1

Actuone PPLO Broth w/o Crystal Violet is an animal-origin free formulation used for the cultivation of Mycoplasma spp. in a laboratory setting. Actuone PPLO Broth w/o Crystal Violet is not intended for use in the diagnosis of disease or other conditions in humans.

Acutone PPLO Broth w/o Crystal Violet was developed for the cultivation of Mycoplasma spp. as an alternative to PPLO Broth w/o CV and is completely free of animal-origin components.

PPLO Broth w/o Crystal Violet is prepared according to the formula described by Morton and Lecce. Acutone PPLO Broth w/o Crystal Violet is a modification of this formula. Crystal Violet may inhibit the growth of Mycoplasma, and therefore was removed from an earlier formula described by Morton, Smith, Williams, and Eikenberg. Mycoplasma was discovered in a case of pleuropneumonia in a cow, and referred to as “pleuropneumonia-like organism” or PPLO. Mycoplasmas belong to the class of Mollicutes “soft skin”, which are the smallest free-living organisms. They are pleomorphic, varying in size from 0.2 to 0.3 micromillimeters.

Specifications
Analyte Mycoplasma spp.
Brand NEOGEN® Culture Media
Formula
Formula Liter
Vegetable Infusion (Dehydrated) 12.5 g/L
Yeast Extract 3.5 g/L
Sodium Chloride 5.0 g/L
Grams per Litre 21.00
Litres per 500g 24.00
Mass 500 g
Package Dimensions 4.60 IN x 2.90 IN x 8.60 IN
Package Weight 1.35 LB
Preparation
  1. Dissolve 21 g of the medium in 700 mL of purified water.
  2. Mix thoroughly.
  3. Autoclave at 121°C for 15 minutes.
  4. Cool to 45 - 50°C and aseptically add 300 mL of the appropriate sterile serum supplement.
  5. Mix thoroughly.
Previous Item Number Product was previously known as Acumedia Product: 7742 Acutone PPLO Broth w/o Crystal Violet

Support

Validation

Our experts are here to help. If you need information beyond what is provided in Validation Reports under the Documents tab, please contact us and we will be happy to assist you. Learn more